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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Young Hwa (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine) Lee, Jae Woong (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine) Chae, Sungwook (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine) Moon, Sang Ho (Division of Food Bioscience, Konkuk University) Do, Eui Jeong (Department of Biological Sciences, Konkuk University) Oh, Seung Eun (Department of Biological Sciences, Konkuk University) Zhang, Gui Jun (School of Chinese Materia Medica, Beijing University of Chinese Medicine) Lee, Mi Young (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제58권 제1호
발행연도
2015.1
수록면
61 - 66 (6page)

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Deer antlers (Cervi Parvum Cornu) are reported to possess various pharmacological properties, including anti-infective, anti-arthritic, anti-allergic, and anti-endometriotic properties, and are credited with reversing memory impairment. To determine the global distribution of Cervus subspecies by analyzing antlers, 166 samples of Cervus subspecies antlers were collected from Russia, Canada, China, New Zealand, and Korea. The respective deer subspecies were identified by amplifying the D-loop region of mitochondrial DNA isolated from the antler samples. On the basis of the mitochondrial DNA sequence, a C. elaphus sibiricus-specific primer was developed that differentiates C. e. sibiricus from four C. e. subspecies (C. e. manitobensis, C. e. nelsoni, C. e. canadensis, and C. e. bactrianus), C. elaphus, and C. nippon. This was confirmed using agarose gel electrophoresis. This primer set produced a specific 396-bp fragment that amplified only C. e. sibiricus in antler samples. A 468-bp fragment derived from the cytochrome b gene was used as the internal control to verify the success of amplification in all samples. This method may assist in rapid and effective differentiation between products from Cervus species and other deer products.

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