지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 133 - 139 (7page)
이용수
초록· 키워드
오류제보하기
DNA methylation is one of the reasons for poor survival of clone animals. The OCT-4 gene is essential for maintaining pluripotency of embryonic stem (ES) cells and early embryos. We previously reported that the 5'-promoter region of Oct-4 gene was a target of DNA methylation and the methylation status was changed variously during embryonic development in bovine. The study conducted to examine the expression and methylation pattern of tissue-dependent differentially methylated region (T-DMR) of Oct-4 gene in bovine somatic cell nuclear transfer (SCNT) and in vitro fertilization (IVF) blastocysts. The Oct-4 gene expression was evaluated by RT-PCR and fluorescence immunocytochemistry. The methylation pattern of T-DMR was analyzed using restriction mapping and bisulfite sequencing methods. The Oct-4 transcripts were highly expressed in IVF, while they were not expressed in SCNT. The Oct-4 protein was not detected or expressed at very low level in SCNT, the intensity of Oct-4 protein, however, was strong in IVF. On the other hand, the T-DMR of Oct-4 gene was hypermethylated in SCNT compared to that of IVF. These results suggested that expression and the failure of demethylation of Oct-4 gene was closely associated with incomplete development of SCNT embryos.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
소 착상 전 초기수정란에서 Oct-4 유전자 Promoter 영역의 DNA 메틸화 변화
한국동물번식학회지
2008 .01
-
Alteration of DNA Methylation in Oct-4 Gene in Mouse Preimplantation Embryos by the Interference RNA
한국동물번식학회지
2007 .01
-
Function of Oct4 in mammalian embryonic development
동물생명과학연구
2015 .06
-
Oct4-Transfection한 중간엽줄기세포 유래 핵이식 배반포의 Oct4 발현 분포 및 세포 자멸사의 변화에 관한 연구
한국수정란이식학회지
2016 .01
-
돼지 체세포복제 35일령 태아에서 H19 메틸화 가변 영역의 DNA 메틸화 변화
한국수정란이식학회지
2011 .01
-
Effect of L-Glutathione Treatment during Somatic Cell Nuclear Transfer Procedures on the Subsequent Embryonic Development and DNA Methylation Status of Cloned Bovine Embryos
한국수정란이식학회지
2014 .01
-
Methylation Pattern of H19 Gene at Various Preimplantation Development Stages of In Vitro Fertilized and Cloned Porcine Embryos
한국동물번식학회지
2007 .01
-
Gene Expression of the In Vitro Fertilized or Somatic Cell Nuclear Transfer Embryos Cultured in Medium Supplemented with Different Proteins or Energy Substrates
한국수정란이식학회지
2010 .01
-
Hypomethylation of DNA in Nuclear Transfer Embryos from Porcine Embryonic Germ Cells
한국수정란이식학회지
2012 .01
-
Factors Affecting the Efficiency of Animal Cloning by Somatic Cell Nuclear Transfer
한국수정란이식학회지
2008 .01
-
Global DNA Methylation of Porcine Embryos during Preimplantation Development
한국가축번식학회지
2003 .01
-
[S3-1] Nutrients and DNA Methylation
한국식품영양과학회 학술대회발표집
2007 .10
-
Control of MPF Activity of Recipient Oocytes and Subsequent Development and DNA Methylation of Somatic Cell Nuclear Transfer Bovine Embryos
한국동물번식학회지
2009 .01
-
Methylation Status of H19 Gene in Embryos Produced by Nuclear Transfer of Spermatogonial Stem Cells in Pig
한국동물번식학회지
2011 .01
-
삼투압 배양 조건에 따른 돼지 체세포 복제 배반포에서 Repeats 영역의 DNA 메틸화 변화
한국동물번식학회지
2010 .01
-
The mRNA Expression and Methylation Pattern of Apoptosis-related and Imprinted Genes in Day 35 of Cloned Pig Fetuses
한국동물번식학회지
2007 .01
-
Comparisons of Development Potential in Bovine SCNT Embryos using Donor Cells treated with Different Demethylating Inhibitors
한국수정란이식학회지
2015 .01
-
돼지 체세포복제 배반포에서 Pre-1 영역의 Non-CpG 메틸화 양상
한국동물번식학회지
2011 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0