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논문 기본 정보

자료유형
학술저널
저자정보
Yeo, Up-Dong (Faculty of Biological Science, College of Natural Sciences, Chonbuk National University) Kim, Kyong-Ho (Faculty of Biological Science, College of Natural Sciences, Chonbuk National University)
저널정보
한국식물학회 식물학회지 식물학회지 제45권 제2호
발행연도
2002.1
수록면
90 - 95 (6page)

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초록· 키워드

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We cultured the suspension cells of kidney bean in MS media supplemented with one of five concentrations of CaCl$_2$[0, 22, 44 (control), 88, or 176 mg/L], and harvested them at the logistic (15 d) and early-stationary (30 d) phases. Cells grown at concentrations higher than 22 mg/L showed better proliferation than those at 0 mg/L. The rate of proliferation also increased with higher concentrations. We fractionated the individual sugars into symplastic (EtOH and starch) and apoplastic (low-molecular pectin, high-molecular pectin, hemicellulose, and cellulose) components. Cells treated at the highest concentration (176 mg/L) exhibited the greatest amount of sugar in the EtOH and starch fraction during the logistic phase. In contrast, cells in the early stationary phase had the highest level of sugar at treatment concentrations of less than 22 mg/L. For treatment concentrations higher than 22mg/L on Day 15, more pectin and hemicellulose was detected at greater amounts compared with those cells treated with 0 mg/L. However, at Day 30, concentrations higher than 44 mg/L induced greater amounts of pectin and hemicellulose than from the other concentrations. Cellulose was more abundant with the 0 mg/L treatment, and contents ranged from 17.4 to 25.5% in the primary cell walls over all treatment concentrations. These results indicate that CaCl$_2$ modulates both symplastic and apoplastic sugar metabolism. Therefore, we suggest that the cell-wall structure may define the mode of polysaccharide biosynthesis during cell growth.

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