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자료유형
학술저널
저자정보
Cho, Jum-Rae (Div. Entomol., Dept. Crop Protec., Natl. Inst. Agric. Scie. Technol., RDA) Kim, Young-Joon (Div. Appl. Biol. Chem., Coll. Agric. Life Sci., Seoul Natl. Univ.) Kim, Jeong-Jun (Div. Entomol., Dept. Crop Protec., Natl. Inst. Agric. Scie. Technol., RDA) Kim, Hong-Sun (Div. Entomol., Dept. Crop Protec., Natl. Inst. Agric. Scie. Technol., RDA) Yoo, Jai-Ki (Div. Entomol., Dept. Crop Protec., Natl. Inst. Agric. Scie. Technol., RDA) Lee, Jeang-Oon (Div. Entomol., Dept. Crop Protec., Natl. Inst. Agric. Scie. Technol., RDA)
저널정보
한국응용곤충학회 Journal of Asia-Pacific Entomology Journal of asia-pacific entomology 제2권 제1호
발행연도
1999.1
수록면
39 - 44 (6page)

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This research was performed to find out and characterize the specific esterase isozymes related to OP and pyrethroid resistance in the tobacco cutworm Spodoptera litura(Fabricius). Two laboratory strains (${DSR}_{5}$ and ${CSR}_{4}$) of S.litura, selected with deltamethrin and chlorpyrifos-methyl, had higher larval esterase activities than Haman-collected(HC) strain. Ten esterase isozymes were separated in the wild HC strain on 6.5% nondenaturing polyacrylamide gel electrophoresis (pH 8.3), but only 5 of them were detected in the two strains selected with insecticides. The isozymes were designated from E1 to E10 according to their mobility to cathode. E4 was stained more strongly in both laboratory-selected strains than in HC strain. The frequency of E2 in ${DSR}_{5}$ strain was higher than in HC strain. E2 and E4 were proved to be arylesterase and carboxyesterase. respectively, according to enzyme inhibition tests. Thus decreased number and increased intensity of esterase bands in ${DSR}_{5}$ and ${CSR}_{4}$ strain may be associated with insecticide resistance, resulting from selections successively with insecticides for several generations.

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