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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 297 - 300 (4page)
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초록· 키워드
오류제보하기
In countries with FMD vaccination, as in Korea, typical clinical signs do not appear, and even in FMD positive cases, it is difficult to isolate the FMDV or obtain whole genome sequence. To overcome this problem, more rapid and simple NGS system is required to control FMD in Korea. FMDV (O/Boeun/ SKR/2017) RNA was extracted and sequenced using Ion Torrent’s bench-top sequencer with amplicon panel with optimized bioinformatics pipelines. The whole genome sequencing of raw data generated data of 1,839,864 (mean read length 283 bp) reads comprising a total of 521,641,058 (≥Q20 475,327,721). Compared with FMDV (GenBank accession No. MG983730), the FMDV sequences in this study show-ed 99.83% nucleotide identity. Further study is needed to identify these differences. In this study, fast and robust methods for benchtop next generation sequencing (NGS) system was developed for analysis of Foot-and-mouth disease virus (FMDV) whole genome sequences.
목차
등록된 정보가 없습니다.
참고문헌 (8)
참고문헌 신청
Alexandersen S / 2003 / The pathogenesis and diagnosis of foot-and-mouth disease / J Comp Path 129:1~36
Alikhan NF / 2011 / BLAST Ring Image Generator(BRIG) : simple prokaryote genome comparisons / BMC Genomics 12:402
Brito BP / 2017 / Review of the global distribution of foot-and-mouth disease virus from 2007 to 2014 / Transbound Emerg Dis 64:316~332
Daum LT / 2012 / Next-generation ion torrent sequencing of drug resistance mutations in Mycobacterium tuberculosis strains / J Clin Microbiol 50:3831~3837
Knowles NJ / 2003 / Molecular epidemiology of footand-mouth disease virus / Virus Res 91:65~80
Alikhan NF / 2011 / BLAST Ring Image Generator(BRIG) : simple prokaryote genome comparisons / BMC Genomics 12:402
Brito BP / 2017 / Review of the global distribution of foot-and-mouth disease virus from 2007 to 2014 / Transbound Emerg Dis 64:316~332
Daum LT / 2012 / Next-generation ion torrent sequencing of drug resistance mutations in Mycobacterium tuberculosis strains / J Clin Microbiol 50:3831~3837
Knowles NJ / 2003 / Molecular epidemiology of footand-mouth disease virus / Virus Res 91:65~80
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