지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Exosomes, known as nanoscale extracellular vesicles in the range of 30–150 nm, are known to contain clinically significant information. However, there is still insufficient information on exosomal membrane proteins for cancer diagnosis. In this work, we investigated the characteristics of the membrane proteins of exosomes shed by cultured breast cancer cell lines using a surface plasmon resonance (SPR) spectroscopy and pre-activated alkanethiols modified sensor chips. The antibodies of breast cancer biomarkers such as MCU-16, EpCAM, CD24, ErbB2, and CA19-9 were immobilized on the pre-activated alkanethiols surfaces without any activation steps. The purified exosomes were loaded onto each antibody surface. The affinity rank of the antibody surfaces was decided by the relative capture efficiency factors for the exosomes. In addition, an antibody with a relative capture efficiency close to 100% was tested with exosome concentration levels of 104 /µl, 105 /µl, and 106 /µl for quantitative analysis.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
C. Lawson / 2016 / Microvesicles and exosomes : new players in metabolic and cardiovascular disease / J. Endocrinol 288:R57~R71
S. H. Jalalian / 2019 / Exosomes, new biomarkers in early cancer detection / Anal. Biochem 571:1~13
L. Console / 2019 / Exosomes in inflammation and role as biomarkers / Clin. Chim. Acta 488:165~171
M. R. Fernando / 2017 / New evidence that a large proportion of human blood plasma cell-free DNA is localized in exosomes / PLoS ONE 12:0183915(1)~0183915(15
T. Pisitkun / 2004 / Identification and proteomic profiling of exosomes in human urine / Proc. Natl. Acad. Sci. U. S. A 101:13368~13373
S. H. Jalalian / 2019 / Exosomes, new biomarkers in early cancer detection / Anal. Biochem 571:1~13
L. Console / 2019 / Exosomes in inflammation and role as biomarkers / Clin. Chim. Acta 488:165~171
M. R. Fernando / 2017 / New evidence that a large proportion of human blood plasma cell-free DNA is localized in exosomes / PLoS ONE 12:0183915(1)~0183915(15
T. Pisitkun / 2004 / Identification and proteomic profiling of exosomes in human urine / Proc. Natl. Acad. Sci. U. S. A 101:13368~13373
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