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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 63 - 67 (5page)
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As 16S ribosomal RNA (rRNA)-targeted sequencing can detect DNA from non-viable bacteria, it can be used to identify pathogens from clinical samples even in patients pretreated with antibiotics. We compared the results of 16S rRNA-targeted sequencing and culture for identifying bacterial species in normally sterile body fluid (NSBF): cerebrospinal, pericardial, peritoneal and pleural fluids. Over a 10-year period, a total of 312 NSBF samples were evaluated simultaneously using 16S rRNA-targeted sequencing and culture. Results were concordant in 287/312 (92.0%) samples, including 277 (88.8%) negative and 10 (3.2%) positive samples. Of the 16 sequencing-positive, culture-negative samples, eight showed clinically relevant isolates that included Fusobacterium nucleatum subsp. nucleatum, Streptococcus pneumoniae, and Staphylococcus spp. All these samples were obtained from the patients pretreated with antibiotics. The diagnostic yield of 16S rRNA-targeted sequencing combined with culture was 11.2%, while that of culture alone was 6.1%. 16S rRNA-targeted sequencing in conjunction with culture could be useful for identifying bacteria in NSBF samples, especially when patients have been pretreated with antibiotics and when anaerobic infection is suspected.
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참고문헌 (20)
참고문헌 신청
Altun O / 2015 / Rapid identification of microorganisms from sterile body fluids by use of FilmArray / J Clin Microbiol 53:710~712
Grif K / 2012 / Improvement of detection of bacterial pathogens in normally sterile body sites with a focus on orthopedic samples by use of a commercial 16S rRNA broad-range PCR and sequence analysis / J Clin Microbiol 50:2250~2254
Rampini SK / 2011 / Broad-range 16S rRNA gene polymerase chain reaction for diagnosis of culture-negative bacterial infections / Clin Infect Dis 53:1245~1251
Sontakke S / 2009 / Use of broad range 16S rDNA PCR in clinical microbiology / J Microbiol Methods 76:217~225
Leber AL / 2016 / Clinical microbiology procedures handbook / ASM Press :3.5~3.7
Grif K / 2012 / Improvement of detection of bacterial pathogens in normally sterile body sites with a focus on orthopedic samples by use of a commercial 16S rRNA broad-range PCR and sequence analysis / J Clin Microbiol 50:2250~2254
Rampini SK / 2011 / Broad-range 16S rRNA gene polymerase chain reaction for diagnosis of culture-negative bacterial infections / Clin Infect Dis 53:1245~1251
Sontakke S / 2009 / Use of broad range 16S rDNA PCR in clinical microbiology / J Microbiol Methods 76:217~225
Leber AL / 2016 / Clinical microbiology procedures handbook / ASM Press :3.5~3.7
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