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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 72 - 75 (4page)
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초록· 키워드
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Accurate detection of BCR-ABL fusion transcripts at and below molecular response (MR) 4 (0.01% International Scale [IS]) is required for disease monitoring in patients with chronic myeloid leukemia (CML). We evaluated the analytical performance of the QXDx BCR-ABL %IS (Bio-Rad, Hercules, CA, USA) droplet digital PCR (ddPCR) assay, which is the first commercially available ddPCR-based in vitro diagnostics product. In precision analysis, the %CV was 9.3% and 3.0%, with mean values of 0.031% IS and 9.4% IS, respectively. The assay was linear in the first order, ranging from 0.032% IS to 20% IS. The manufacturer-claimed limit of blank, limit of detection, and limit of quantification were verified successfully. There was a very strong correlation between the results of the QXDx BCR-ABL %IS ddPCR assay and the ipsogen BCR-ABL1 Mbcr IS-MMR (Qiagen, Hilden, Germany) real-time quantitative PCR assay (r=0.996). In conclusion, the QXDx BCR-ABL %IS ddPCR assay can provide reliable results for CML patients.
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참고문헌 (18)
참고문헌 신청
Baccarani M / 2015 / A review of the European LeukemiaNet recommendations for the management of CML / Ann Hematol 94:S141~S147
Radich JP / 2018 / Chronic myeloid leukemia, version 1.2019, NCCN clinical practice guidelines in oncology / J Natl Compr Canc Netw 16:1108~1135
Branford S / 2006 / Rationale for the recommendations for harmonizing current methodology for detecting BCR-ABL transcripts in patients with chronic myeloid leukaemia / Leukemia 20:1925~1930
Jennings LJ / 2014 / Detection and quantification of BCR-ABL1 fusion transcripts by droplet digital PCR / J Mol Diagn 16:174~179
Kong JH / 2017 / Does the frequency of molecular monitoring after tyrosine kinase inhibitor discontinuation affect outcomes of patients with chronic myeloid leukemia? / Cancer 123:2482~2488
Radich JP / 2018 / Chronic myeloid leukemia, version 1.2019, NCCN clinical practice guidelines in oncology / J Natl Compr Canc Netw 16:1108~1135
Branford S / 2006 / Rationale for the recommendations for harmonizing current methodology for detecting BCR-ABL transcripts in patients with chronic myeloid leukaemia / Leukemia 20:1925~1930
Jennings LJ / 2014 / Detection and quantification of BCR-ABL1 fusion transcripts by droplet digital PCR / J Mol Diagn 16:174~179
Kong JH / 2017 / Does the frequency of molecular monitoring after tyrosine kinase inhibitor discontinuation affect outcomes of patients with chronic myeloid leukemia? / Cancer 123:2482~2488
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