Evaluation of a new flow cytometry based method for detection of BCR-ABL1 fusion protein in chronic myeloid leukemia

Swati Dasgupta; Ujjal K Ray; Arpita Ghosh Mitra; Deboshree M. Bhattacharyya; Ashis Mukhopadhyay; Priyabrata Das; Sudeshna Gangopadhyay; Sudip Roy; Soma Mukhopadhyay

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저자정보: Swati Dasgupta (Medica Superspeciality Hospital) Ujjal K Ray (Medica Superspeciality Hospital) Arpita Ghosh Mitra (Medica Superspeciality Hospital) Deboshree M. Bhattacharyya (Medica Superspeciality Hospital) Ashis Mukhopadhyay (Medica Superspeciality Hospital) Priyabrata Das (Medica Superspeciality Hospital) Sudeshna Gangopadhyay (Medica Superspeciality Hospital) Sudip Roy (Medica Superspeciality Hospital) Soma Mukhopadhyay (Medica Superspeciality Hospital)

저널정보: 대한혈액학회 Blood Research Blood Research Vol.52 No.2

발행연도: 2017.1

수록면: 112 - 118 (7page)

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Background: Philadelphia chromosome, a hallmark of chronic myeloid leukemia (CML), plays a key role in disease pathogenesis. It reflects a balanced reciprocal translocation between long arms of chromosomes 9 and 22 involving BCR and ABL1 genes, respectively. An accurate and reliable detection of BCR-ABL fusion gene is necessary for the diagnosis and monitor-ing of CML. Previously, many technologies, most of which are laborious and time consum-ing, have been developed to detect BCR-ABL chimeric gene or chromosome. Methods: A new flow cytometric immunobead assay was used for detection of BCR-ABL fusion pro-teins and applicability, sensitivity, reliability, efficacy and rapidity of this method was evaluated. Results: From February 2009 to January 2014, a total 648 CML patients were investigated for the status of BCR-ABL1 protein. Among them, 83 patients were enrolled for comparative study of BCR-ABL1 positivity by three routinely used procedures like karyotyping, and quantita-tive real time PCR (RT-PCR) as well as immunobead flow cytometry assay. BCR-ABL protein analysis was found consistent, more sensitive (17% greater sensitivity) and reliable than the conventional cytogenetics, as flow cytometry showed 95% concordance rate to RT-PCR. Conclusion: BCR-ABL fusion protein assay using a new flow cytometric immunobead might be useful in the diagnosis and monitoring CML patients.

참고문헌 (26)

참고문헌 신청

Dasgupta S / 2013 / An accurate and rapid flowcytomertic diagnosis of BCR ABL fusion protein an alternative way to detect the different phases of chronic myeloid leukemia / Int J Adv Scientific Tech Res 3:195~207

Heisterkamp N / 1983 / Localization of the c-ab1 oncogene adjacent to a translocation break point in chronic myelocytic leukaemia / Nature 306:239~242

Rowley JD / 1973 / Letter : A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining / Nature 243:290~293

Chasseriau J / 2004 / Characterization of the different BCR-ABL transcripts with a single multiplex RT-PCR / J Mol Diagn 6:343~347

Raponi S / 2009 / An accurate and rapid flow cytometric diagnosis of BCR-ABL positive acute lymphoblastic leukemia / Haematologica 94:1767~1770

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