지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제24권 제1호
- 발행연도
- 2020.1
- 수록면
- 81 - 88 (8page)
이용수
초록· 키워드
오류제보하기
Regulator of calcineurin 1 (RCAN1) can be induced by an intracellular calcium increase and oxidative stress, which are characteristic features of temporal lobe epilepsy. Thus, we investigated the spatiotemporal expression and cellular localization of RCAN1 protein and mRNA in the mouse hippocampus after pilocarpine-induced status epilepticus (SE). Male C57BL/6 mice were given pilocarpine hydrochloride (280 mg/kg, i.p.) and allowed to develop 2 h of SE. Then the animals were given diazepam (10 mg/kg, i.p.) to stop the seizures and sacrificed at 1, 3, 7, 14, or 28 day after SE. Cresyl violet staining showed that pilocarpine-induced SE resulted in cell death in the CA1 and CA3 subfields of the hippocampus from 3 day after SE. RCAN1 immunoreactivity showed that RCAN1 was mainly expressed in neurons in the shammanipulated hippocampi. At 1 day after SE, RCAN1 expression became detected in hippocampal neuropils. However, RCAN1 signals were markedly enhanced in cells with stellate morphology at 3 and 7 day after SE, which were confirmed to be reactive astrocytes, but not microglia by double immunofluorescence. In addition, realtime reverse transcriptase–polymerase chain reaction showed a significant upregulation of RCAN1 isoform 4 (RCAN1-4) mRNA in the SE-induced hippocampi. Finally, in situ hybridization with immunohistochemistry revealed astrocytic expression of RCAN1-4 after SE. These results demonstrate astrocytic upregulation of RCAN1 and RCAN1-4 in the mouse hippocampus in the acute and subacute phases of epileptogenesis, providing foundational information for the potential role of RCAN1 in reactive astrocytes during epileptogenesis.
목차
등록된 정보가 없습니다.
참고문헌 (38)
참고문헌 신청
Fisher RS / 2014 / ILAE official report : a practical clinical definition of epilepsy / Epilepsia 55:475~482
Blair RD / 2012 / Temporal lobe epilepsy semiology / Epilepsy Res Treat 2012:751510
Becker AJ / 2018 / Review : animal models of acquired epilepsy : insights into mechanisms of human epileptogenesis / Neuropathol Appl Neurobiol 44:112~129
Hoeffer CA / 2007 / The Down syndrome critical region protein RCAN1 regulates long-term potentiation and memory via inhibition of phosphatase signaling / J Neurosci 27:13161~13172
Porta S / 2007 / Differential expression of members of the RCAN family of calcineurin regulators suggests selective functions for these proteins in the brain / Eur J Neurosci 26:1213~1226
Blair RD / 2012 / Temporal lobe epilepsy semiology / Epilepsy Res Treat 2012:751510
Becker AJ / 2018 / Review : animal models of acquired epilepsy : insights into mechanisms of human epileptogenesis / Neuropathol Appl Neurobiol 44:112~129
Hoeffer CA / 2007 / The Down syndrome critical region protein RCAN1 regulates long-term potentiation and memory via inhibition of phosphatase signaling / J Neurosci 27:13161~13172
Porta S / 2007 / Differential expression of members of the RCAN family of calcineurin regulators suggests selective functions for these proteins in the brain / Eur J Neurosci 26:1213~1226
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
A pilocarpine-induced animal model for pathogenesis and treatment of Temporal Lobe Epilepsy
한국실험동물학회 학술발표대회 논문집
2021 .07
-
Time-course changes of hippocalcin expression in the mouse hippocampus following pilocarpine-induced status epilepticus
Journal of Veterinary Science
2016 .06
-
Neuroprotective effect of lithium after pilocarpine-induced status epilepticus in mice
The Korean Journal of Physiology & Pharmacology
2017 .01
-
Increased expression of vascular endothelial growth factor-C and vascular endothelial growth factor receptor-3 after pilocarpineinduced status epilepticus in mice
The Korean Journal of Physiology & Pharmacology
2019 .01
-
The correlation of neuronal disorder with the alterations of Phospholipase C (PLC) beta expressionin the hippocampus following pilocarpine-induced status epilepticus
대한체질인류학회 학술대회 연제 초록
2015 .05
-
Expression of calcium-binding protein positive neurons in the main olfactory bulb following lithium pilocarpine-induced status epilepticus in rat
대한체질인류학회 학술대회 연제 초록
2016 .05
-
Comparison of the Effects of Pilocarpine Solution and Tablet on Salivary Flow Rate
Journal of Oral Medicine and Pain
2015 .03
-
Analysis of Correlation between Thyroid Cancer Incidence and Socioeconomic Status Using 10-year Sample Cohort Database
The Journal of Endocrine Surgery
2019 .01
-
Effect of parvalbumin-positive neurons in the main olfactory bulb following lithium pilocarpine-induced status epilepticus in rat
대한체질인류학회 학술대회 연제 초록
2016 .05
-
NOX4 deficiency lowers the neuronal differentiation and severely reduces the population of astrocytes in the hippocampus
한국실험동물학회 학술발표대회 논문집
2020 .07
-
Clinical Characteristics and Weather Effects on Pediatric Status Epilepticus Patients Visiting the Emergency Room
대한소아신경학회지
2016 .09
-
The correlation of muscarinic acetylcholine 1 receptor with status epilepticus in the hippocampus
대한체질인류학회 학술대회 연제 초록
2017 .06
-
Effect of Individual and District-level Socioeconomic Disparities on Cognitive Decline in Community-dwelling Elderly in Seoul
Journal of Korean Medical Science
2017 .01
-
NOX4 affects the stage of neural differentiation in the hippocampus through independent with an inflammatory contribution
한국실험동물학회 학술발표대회 논문집
2020 .02
-
Alteration of calcium-binding protein expressions in hippocampus of the traumatic brain injury
대한체질인류학회 학술대회 연제 초록
2016 .05
-
Association between Obesity and Neighborhood Socioeconomic Status in Korean Adolescents Based on the 2013 Korea Youth Risk Behavior Web-Based Survey
Korean Journal of Family Medicine
2016 .01
-
The sequelae of behavioral phenotypes following abnormal efficacies of hippocampal circuits in the febrile seizure
대한체질인류학회 학술대회 연제 초록
2021 .05
-
Anti-oxidative effect of baicalin in lipopolysaccharide-induced hippocampus damage
예방수의학회지
2020 .01
-
The functional relevance of muscarinic acetylcholine receptor and Phospholipase C (PLC) beta expression following status epilepticus in the hippocampus
대한체질인류학회 학술대회 연제 초록
2015 .05
-
Differential gene expression in the hippocampus of SREBP1c knockout mice
한국실험동물학회 학술발표대회 논문집
2020 .02
이 논문의 저자 정보
최근 본 자료
댓글(0)
0