Uncertainty Minimization in Quantitative Electron Spin Resonance Measurement: Considerations on Sampling Geometry and Signal Processing

Sangeon-Park; 심정현; 김기웅; 정근홍; 송남웅

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자료유형: 학술저널

저자정보: Sangeon-Park 심정현 김기웅 정근홍 송남웅

저널정보: 한국자기공명학회 Journal of the Korean Magnetic Resonance Society Journal of the Korean Magnetic Resonance Society 제24권 제2호

발행연도: 2020.1

수록면: 53 - 58 (6page)

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Free radicals including reactive oxygen species (ROS) are important chemicals in the research area of biology, pharmaceutical, medical, and environmental science as well as human health risk assessment as they are highly involved in diverse metabolism and toxicity mechanisms through chemical reactions with various components of living bodies. Electron spin resonance (ESR) spectroscopy is a powerful tool for detecting and quantifying those radicals in biological environments. In this work we observed the ESR signal of 2,2,6,6-Tetra-methyl piperidine 1-oxyl (TEMPO) in aqueous solution at various concentrations to estimate the uncertainty factors arising from the experimental conditions and signal treatment methods. As the sample position highly influences the signal intensity, dual ESR tube geometry (consists of a detachable sample tube and a position fixed external tube) was adopted. This type of measurement geometry allowed to get the relative uncertainty of signal intensity lower than 1% when triple measurements are averaged. Linear dependence of signal intensity on the TEMPO concentration, which is required for the quantification of unknown sample, could be obtained over a concentration range of ~103 by optimizing the signal treatment method depending on the concentration range.

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참고문헌 신청

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P. Pacher / 2007 / Physiol. Rev 87:315

J. M. Mccords / 1969 / J. Biol. Chem 244:6049

I. Juránek / 2013 / Food Chem. Toxicol 61:240

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