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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제4호
- 발행연도
- 2020.1
- 수록면
- 552 - 560 (9page)
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초록· 키워드
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Human carbonic anhydrase (CA) isozyme II has been used as protein target for disorder treatment including glaucoma. Current clinically used sulfonamide-based CA inhibitors can induce side effects, and so alternatives are required. This study aimed to investigate a natural CA inhibitor from Murraya paniculata. The previously developed yeast-based assay was used to screen 14 compounds isolated from M. paniculata and identified by NMR analysis for anti-human CA isozyme II (hCAII) activity. Cytotoxicity of the compounds was also tested using the same yeast-based assay but in a different cultivation condition. Two flavonoid candidate compounds, 5, 6, 7, 8, 3’, 4’, 5’- heptamethoxyflavone (4) and 3 ,5, 7, 8, 3’, 4’, 5’-heptamethoxyflavone (9), showed potent inhibitory activity against hCAII with a minimal effective concentration of 10.8 and 21.5 μM, respectively, while they both exhibited no cytotoxic effect, even at the highest concentration tested (170 μM). The results from an in vitro esterase assay of the two candidates confirmed their hCAII inhibitory activity with IC50 values of 24.0 and 34.3 μM, respectively. To investigate the potential inhibition mechanism of compound 4, in silico molecular docking was performed using the FlexX and SwissDock software. This revealed that compound 4 coordinated with the Zn2+ ion in the hCAII active site through its methoxy oxygen at a distance of 1.60 Å (FlexX) or 2.29 Å (SwissDock). The interaction energy of compound 4 with hCAII was -13.36 kcal/mol. Thus, compound 4 is a potent novel flavonoid-based hCAII inhibitor and may be useful for further anti-CAII design and development.
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참고문헌 (63)
참고문헌 신청
Song P / 2017 / National and subnational prevalence and burden of glaucoma in China : a systematic analysis / J. Glob. Health 7:020705
Liang YB / 2017 / Proposing new indicators for glaucoma healthcare service / Eye Vis. (Lond) 4:6
Varma R / 2011 / An assessment of the health and economic burdens of glaucoma / Am. J. Ophthalmol 152:515~522
Goel M / 2010 / Aqueous humor dynamics : a review / Open Ophthalmol. J 4:52~59
Sly WS / 1995 / Human carbonic anhydrases and carbonic anhydrase deficiencies / Annu. Rev. Biochem 64:375~401
Liang YB / 2017 / Proposing new indicators for glaucoma healthcare service / Eye Vis. (Lond) 4:6
Varma R / 2011 / An assessment of the health and economic burdens of glaucoma / Am. J. Ophthalmol 152:515~522
Goel M / 2010 / Aqueous humor dynamics : a review / Open Ophthalmol. J 4:52~59
Sly WS / 1995 / Human carbonic anhydrases and carbonic anhydrase deficiencies / Annu. Rev. Biochem 64:375~401
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