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논문 기본 정보

자료유형
학술저널
저자정보
Jianping Liang (Shanxi Agricultural University) Wenqian Li (Shanxi Agricultural University) Xiaoyun Jia (Shanxi Agricultural University) Ying Zhang (Shanxi Agricultural University) Jianping Zhao (Shanxi University of Chinese Medicine)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.42 No.8
발행연도
2020.1
수록면
901 - 914 (14page)

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Background Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao is a traditional medicinal herb of Leguminosae since it contains bioactive compounds such as flavonoids, which have significant pharmacological effects on immunity and antioxidant. However, the scanty genomic and transcriptome resources of Astragalus membranaceus have hindered further exploration of its biosynthesis and accumulation mechanism. Objective This project aim to further improve our understanding of the relationship between transcriptional behavior and flavonoids content of A. mongholicus. Methods The accumulation of flavonoids and related gene expression in five different developmental stages (A: vegetative, B: florescence, C: fruiting, D: fruit ripening and E: defoliating stages) of A. mongholicus root were studied by combining UV spectrophotometry and transcriptomic techniques. The de novo assembly, annotation and functional evaluation of the contigs were performed with bioinformatics tools. Results After screening and assembling the raw data, there were a total of 158,123 unigenes with an average length of 644.89 bp were finally obtained, which has 8362 unigenes could be jointly annotated by NR, SwissProt, eggNOG, GO, KEGG and Pfam databases. KEGG enrichment analysis was performed on differentially expressed genes(DEGs)in the four groups (A vs. B, B vs. C, C vs. D, D vs. E). The results showed that many DEGs in each group were significantly enriched to flavonoids biosynthesis related pathways. Among them, a number of 86 were involved in the biosynthesis of isoflavonoid (12), flavonoid (5) and phenylpropanoid (69). Further analysis of these DEGs revealed that the expression levels of key genes such as PAL, 4CL, CCR , COMT, DFR, etc. were all down-regulated at the fruiting stage, and then raised at the fruit ripening stage. This expression pattern was similar to the accumulation trend of total flavonoids content. Conclusions In summary, this comprehensive transcriptome dataset allowed the identification of genes associated with flavonoids metabolic pathways. The results laid a foundation for the biosynthesis and regulation of flavonoids. It also provided a scientific basis for the most suitable harvest time and resource utilization of A. mongholicus.
#Astragalus mongholicus · Developmental stages · Transcriptome sequencing · qRT-PCR · Flavonoids

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참고문헌 (32)

참고문헌 신청
Chen J / 2015 / Global transcriptome analysis profiles metabolic pathways in traditional herb Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao / BMC Genom 16(Suppl 7):S15 google schola Cheng ZC / 2018 / Characterization of moso bamboo(Phyllostachys edulis)Dof factors in floral development and abiotic stress responses / Genome 61:151~156 google schola Czemmel S / 2012 / R2r3 myb transcription factors : key regulators of the flavonoid biosynthetic pathway in grapevine / Protoplasma 249:109~118 google schola Gao W / 2014 / Combining metabolomics and transcriptomics to characterize tanshinone biosynthesis in Salvia miltiorrhiza / BMC Genom 15:73 google schola Kim YB / 2014 / Accumulation of astragalosides and related gene expression in different organs of Astragalus Membranaceus Bge / Var Mongholicus(Bge)Molecules 19:10922~10935 google schola

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