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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제10호
- 발행연도
- 2019.1
- 수록면
- 1,656 - 1,664 (9page)
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초록· 키워드
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Isoprene has the potential to replace some petroleum-based chemicals and can be produced through biological systems using renewable carbon sources. Ralstonia eutropha can produce value-added compounds, including intracellular polyhydroxyalkanoate (PHA) through fatty acid and lipid metabolism. In the present study, we engineered strains of R. eutropha H16 and examined the strains for isoprene production. We optimized codons of all the genes involved in isoprene synthesis by the mevalonate pathway and manipulated the promoter regions using pLac and pJ5 elements. Our results showed that isoprene productivity was higher using the J5 promoter (1.9 ± 0.24 μg/l) than when using the lac promoter (1.5 ± 0.2 μg/l). Additionally, the use of three J5 promoters was more efficient (3.8 ± 0.18 μg/l) for isoprene production than a one-promoter system, and could be scaled up to a 5-L batch-cultivation from a T-flask culture. Although the isoprene yield obtained in our study was insufficient to meet industrial demands, our study, for the first time, shows that R. eutropha can be modified for efficient isoprene production and lays the foundation for further optimization of the fermentation process.
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참고문헌 (36)
참고문헌 신청
Lee WH / 2008 / Biosynthesis of polyhydroxyalkanoate copolymers from mixtures of plant oils and 3-hydroxyvalerate precursors / Bioresour. Technol 99:6844~6851
Budde CF / 2011 / Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains / Appl. Environ. Microbiol 77:2847~2854
Doi Y / 1989 / Nuclear magnetic resonance studies of poly(3-Hydroxybutyrate)and polyphosphate metabolism in alcaligenes eutrophus / Appl. Environ. Microbiol 55:2932~2938
Tanaka K / 1995 / Production of poly(D-3-hydroxybutyrate) from CO(2), H(2), and O(2) by high cell density autotrophic cultivation of Alcaligenes eutrophus / Biotechnol. Bioeng 45:268~275
Park JM / 2011 / Genome-scale reconstruction and in silico analysis of the Ralstonia eutropha H16 for polyhydroxyalkanoate synthesis, lithoautotrophic growth, and 2-methyl citric acid production / BMC Syst. Biol 5:101
Budde CF / 2011 / Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains / Appl. Environ. Microbiol 77:2847~2854
Doi Y / 1989 / Nuclear magnetic resonance studies of poly(3-Hydroxybutyrate)and polyphosphate metabolism in alcaligenes eutrophus / Appl. Environ. Microbiol 55:2932~2938
Tanaka K / 1995 / Production of poly(D-3-hydroxybutyrate) from CO(2), H(2), and O(2) by high cell density autotrophic cultivation of Alcaligenes eutrophus / Biotechnol. Bioeng 45:268~275
Park JM / 2011 / Genome-scale reconstruction and in silico analysis of the Ralstonia eutropha H16 for polyhydroxyalkanoate synthesis, lithoautotrophic growth, and 2-methyl citric acid production / BMC Syst. Biol 5:101
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