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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 850 - 857 (8page)
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초록· 키워드
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The Gram-negative opportunistic pathogen, Pseudomonas aeruginosa, has multiple multidrug efflux pumps. MexT, a LysR-type transcriptional regulator, functions as a transcriptional activator of the MexEF-OprN efflux system. MexT consists of an N-terminal DNA‑binding domain and a C‑terminal regulatory domain (RD). Little is known regarding MexT ligands and its mechanism of activation. We elucidated the crystal structure of the MexT RD at 2.0 Å resolution. The structure comprised two protomer chains in a dimeric arrangement. MexT possessed an arginine-rich region and a hydrophobic patch lined by a variable loop, both of which are putative ligand‑binding sites. The three-dimensional structure of MexT provided clues to the interacting ligand structure. A DNase I footprinting assay of full-length MexT identified two MexT-binding sequence in the mexEF-oprN promoter. Our findings enhance the understanding of the regulation of MexT-dependent activation of efflux pumps.
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참고문헌 (34)
참고문헌 신청
Adams, P. D. / 2010 / PHENIX: a comprehensive Python-based system for macromolecular structure solution / Acta Crystallogr. D Biol. Crystallogr 66(Pt 2):213~221
Choi, H. / 2001 / Structural basis of the redox switch in the OxyR transcription factor / Cell 105:103~113
Dallakyan, S. / 2015 / Small-molecule library screening by docking with PyRx / Methods Mol. Biol 1263:243~250
Emsley, P. / 2010 / Features and development of Coot. Acta Crystallogr / D Biol. Crystallogr 66(Pt 4):486~501
Fargier, E. / 2012 / MexT functions as a redox-responsive regulator modulating disulfide stress resistance in Pseudomonas aeruginosa / J. Bacteriol 194:3502~3511
Choi, H. / 2001 / Structural basis of the redox switch in the OxyR transcription factor / Cell 105:103~113
Dallakyan, S. / 2015 / Small-molecule library screening by docking with PyRx / Methods Mol. Biol 1263:243~250
Emsley, P. / 2010 / Features and development of Coot. Acta Crystallogr / D Biol. Crystallogr 66(Pt 4):486~501
Fargier, E. / 2012 / MexT functions as a redox-responsive regulator modulating disulfide stress resistance in Pseudomonas aeruginosa / J. Bacteriol 194:3502~3511
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