지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.2
- 수록면
- 180 - 186 (7page)
- DOI
- 10.14348/molcells.2015.2277
이용수
초록· 키워드
오류제보하기
Escherichia coli AcrAB-TolC is a multidrug efflux pump that expels a wide range of toxic substrates. The dynamic nature of the binding or low affinity between the components has impeded elucidation of how the three components assemble in the functional state. Here, we created fusion proteins composed of AcrB, a transmembrane linker, and two copies of AcrA. The fusion protein exhibited acridine pumping activity, suggesting that the protein reflects the functional structure in vivo. To discern the assembling mode with TolC, the AcrBA fusion protein was incubated with TolC or a chimeric protein containing the TolC aperture tip region. Three-dimensional structures of the complex proteins were determined through transmission electron microscopy. The overall structure exemplifies the adaptor bridging model, wherein the funnel-like AcrA hexamer forms an intermeshing cogwheel interaction with the -barrel tip region of TolC, and a direct interaction between AcrB and TolC is not allowed. These observations provide a structural blueprint for understanding multidrug resistance in pathogenic Gram-negative bacteria.
목차
등록된 정보가 없습니다.
참고문헌 (34)
참고문헌 신청
Du, D. / 2014 / Structure of the AcrAB-TolC multidrug efflux pump / Nature 509:512~515
Emsley, P. / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr. D Biol. Crystallogr 60:2126~2132
Fralick, J. A. / 1996 / Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli / J. Bacteriol 178:5803~5805
Goddard, T. D. / 2007 / Visualizing density maps with UCSF Chimera / J. Struct. Biol 157:281~287
Hinchliffe, P. / 2013 / Structure and operation of bacterial tripartite pumps / Annu. Rev. Microbiol 67:221~242
Emsley, P. / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr. D Biol. Crystallogr 60:2126~2132
Fralick, J. A. / 1996 / Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli / J. Bacteriol 178:5803~5805
Goddard, T. D. / 2007 / Visualizing density maps with UCSF Chimera / J. Struct. Biol 157:281~287
Hinchliffe, P. / 2013 / Structure and operation of bacterial tripartite pumps / Annu. Rev. Microbiol 67:221~242
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