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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.6
- 수록면
- 50 - 58 (9page)
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초록· 키워드
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Sphacelaria is a filamentous brown algal genus that can be epibiotic on macroalgae, marine plants, and sea turtles. Its important role in benthic ecosystems, exposure to different stressors (e.g., grazing), and use as a model organism make Sphacelaria ideal for assessing physiological responses of organisms to environmental inputs. Single-cell RNA sequencing is a powerful new probe for understanding environmental responses of organisms at the molecular (transcriptome) level, capable of delineating gene regulation in different cell types. In the case of plants, this technique requires protoplasts (“naked” plant cells). The existing protoplast isolation protocols for Sphacelaria use non-commercial enzymes and are low-yielding. This study is the first to report the production of protoplasts from Sphacelaria fusca (Hudson) S.F. Gray, using a combination of commercial enzymes, chelation, and osmolarity treatment. A simple combination of commercial enzymes (cellulase Onozuka RS, alginate lyase, and driselase) with chelation pretreatment and an increased osmolarity (2512 mOsm/L H₂O) gave a protoplast yield of 15.08 ± 5.31 × 10⁴ protoplasts/g fresh weight, with all the Sphacelaria cell types represented. Driselase had no crucial effect on the protoplast isolation. However, the increased osmolarity had a highly significant and positive effect on the protoplast isolation, and chelation pretreatment was essential for optimal protoplast yield. The protocol represents a significant step forward for studies on Sphacelaria by efficiently generating protoplasts suitable for cellular studies, including single-cell RNA sequencing and expression profiling.
목차
Abstract
Introduction
Materials and methods
Results
Discussion
Conclusion
References
참고문헌 (54)
참고문헌 신청
Piazzi, L. / 2015 / Epiphyte assemblages of the Mediterranean seagrass Posidonia oceanica : an overview / Mar. Ecol 37:3~41
Varisco, M. / 2015 / Food and habitat choice in the spider crab Leucippa pentagona(Majoidea : Epialtidae)in Bahía Bustamante, Patagonia, Argentina / Scientia Marina 79:107~116
Velasco-Charpentier, C. / 2016 / Epibiota of juvenile Hawksbill Sea Turtles Eretmochelys imbricata stranded in the coast of Rocha Department, Uruguay / Rev. Biol. Mar. Oceanogr 51:449~453
Pavia, H. / 1999 / Habitat and feeding preferences of crustacean mesoherbivores inhabiting the brown seaweed Ascophyllum nodosum (L.)Le Jol. and its epiphytic macroalgae / J. Exp. Mar. Biol. Ecol. 236:15~32
Karez, R. / 2000 / ‘Coconsumption’and ‘protective coating’: two new proposed effects of epiphytes on their macroalgal hosts in mesograzer‐epiphyte‐host interactions / Mar. Ecol. Prog. Ser. 205:85~93
Varisco, M. / 2015 / Food and habitat choice in the spider crab Leucippa pentagona(Majoidea : Epialtidae)in Bahía Bustamante, Patagonia, Argentina / Scientia Marina 79:107~116
Velasco-Charpentier, C. / 2016 / Epibiota of juvenile Hawksbill Sea Turtles Eretmochelys imbricata stranded in the coast of Rocha Department, Uruguay / Rev. Biol. Mar. Oceanogr 51:449~453
Pavia, H. / 1999 / Habitat and feeding preferences of crustacean mesoherbivores inhabiting the brown seaweed Ascophyllum nodosum (L.)Le Jol. and its epiphytic macroalgae / J. Exp. Mar. Biol. Ecol. 236:15~32
Karez, R. / 2000 / ‘Coconsumption’and ‘protective coating’: two new proposed effects of epiphytes on their macroalgal hosts in mesograzer‐epiphyte‐host interactions / Mar. Ecol. Prog. Ser. 205:85~93
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UCI(KEPA) : I410-ECN-0101-2020-472-000688291