지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.1
- 수록면
- 1 - 6 (6page)
이용수
초록· 키워드
오류제보하기
Genome editing has developed rapidly in various research fields for targeted genome modifications in many organisms, including cells, plants, viruses, and animals. The clustered regularly interspaced short palindromic repeats-associated protein 9 system stands as a potent tool in gene editing for generating cells and animal models with high precision. The clinical potential of clustered regularly interspaced short palindromic repeats-associated protein 9 has been extensively reported, with applications in genetic disease correction, inhibition of viral replication, and personalized or targeted therapeutics for various cancers. In this study, we provide a guide on single-guide RNA design, cloning single-guide RNA into plasmid vectors, single-cell isolation via transfection, and identification of knockout clones using next-generation sequencing. In addition, by providing the results of insertion into mammalian cell lines through next-generation sequencing, we offer useful information to those conducting research on human and animal cell lines.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Multiplexed multimodal single-cell technologies: From observation to perturbation analysis
Molecules and Cells
2024 .01
-
Single-Cell Toolkits Opening a New Era for Cell Engineering
Molecules and Cells
2021 .01
-
Genome editing using CRISPR, CAST, and Fanzor systems
Molecules and Cells
2024 .01
-
Brief guide to senescence assays using cultured mammalian cells
Molecules and Cells
2024 .01
-
Single-Cell Molecular Barcoding to Decode Multimodal Information Defining Cell States
Molecules and Cells
2023 .02
-
Unlocking biological mechanisms with integrative functional genomics approaches
Molecules and Cells
2024 .01
-
Unleashing the Therapeutic Potential of CAR-T Cell Therapy Using Gene-Editing Technologies
Molecules and Cells
2018 .08
-
RNA in Therapeutics: CRISPR in the Clinic
Molecules and Cells
2023 .01
-
Single-Cell Analysis: Technology, Data Analysis, and Application
Molecules and Cells
2023 .02
-
Mammalian Cell Culture Process Optimization Using Design of Experiment
한국미생물학회 학술대회논문집
2022 .10
-
The CRISPR Growth Spurt: from Bench to Clinic on Versatile Small RNAs
Journal of Microbiology and Biotechnology
2017 .01
-
Single-Cell Genomics for Investigating Pathogenesis of Inflammatory Diseases
Molecules and Cells
2023 .02
-
Efficient CRISPR-Cas12f1-Mediated Multiplex Bacterial Genome Editing via Low-Temperature Recovery
Journal of Microbiology and Biotechnology
2024 .07
-
Dissecting Cellular Heterogeneity Using Single-Cell RNA Sequencing
Molecules and Cells
2019 .01
-
Ovarian transcriptome profiles associated with sexual maturation in Pacific abalone (Haliotis discus hannai)
Genes & Genomics
2020 .01
-
Non-viral CRISPR/Cas9 based gene editing for liver related diseases
한국분석과학회 학술대회
2022 .05
-
The Workflow for Computational Analysis of Single-cell RNA-sequencing Data
대한임상검사과학회지
2024 .03
-
Inhibition of cell growth by cellular differentiation into adipocyte-like cells in dexamethasone sensitive cancer cell lines
Animal cells and systems
2018 .01
-
Colorimetric Virus Detection Using CRISPR/dCas9
한국진공학회 학술발표회초록집
2021 .02
-
Recent trends in CRISPR‑Cas system: genome, epigenome, and transcriptome editing and CRISPR delivery systems
Genes & Genomics
2019 .01
최근 본 자료
댓글(0)
0