지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 161 - 167 (7page)
이용수
초록· 키워드
오류제보하기
Background: The ascomycete fungi Cylindrocarpon destructans (Cd) and Fusarium solani (Fs) cause ginseng root rot and significantly reduce the quality and yield of ginseng. Cd produces the secondary metabolite radicicol, which targets the molecular chaperone Hsp90. Fs is resistant to radicicol, whereas other fungal genera associated with ginseng disease are sensitive to it. Radicicol resistance mechanisms have not yet been elucidated.
Methods: Transcriptome analyses of Fs and Cd mycelia treated with or without radicicol were conducted using RNA-seq. All of the differentially expressed genes (DEGs) were functionally annotated using the Fusarium graminearum transcript database. In addition, deletions of two transporter genes identified by RNA-seq were created to confirm their contributions to radicicol resistance.
Results: Treatment with radicicol resulted in upregulation of chitin synthase and cell wall integrity genes in Fs and upregulation of nicotinamide adenine dinucleotide dehydrogenase and sugar transporter genes in Cd. Genes encoding an ATP-binding cassette transporter, an aflatoxin efflux pump, ammonium permease 1 (mep1), and nitrilase were differentially expressed in both Fs and Cd. Among these four genes, only the ABC transporter was upregulated in both Fs and Cd. The aflatoxin efflux pump and mep1 were upregulated in Cd, but downregulated in Fs, whereas nitrilase was down-regulated in both Fs and Cd.
Conclusion: The transcriptome analyses suggested radicicol resistance pathways, and deletions of the transporter genes indicated that they contribute to radicicol resistance.
Methods: Transcriptome analyses of Fs and Cd mycelia treated with or without radicicol were conducted using RNA-seq. All of the differentially expressed genes (DEGs) were functionally annotated using the Fusarium graminearum transcript database. In addition, deletions of two transporter genes identified by RNA-seq were created to confirm their contributions to radicicol resistance.
Results: Treatment with radicicol resulted in upregulation of chitin synthase and cell wall integrity genes in Fs and upregulation of nicotinamide adenine dinucleotide dehydrogenase and sugar transporter genes in Cd. Genes encoding an ATP-binding cassette transporter, an aflatoxin efflux pump, ammonium permease 1 (mep1), and nitrilase were differentially expressed in both Fs and Cd. Among these four genes, only the ABC transporter was upregulated in both Fs and Cd. The aflatoxin efflux pump and mep1 were upregulated in Cd, but downregulated in Fs, whereas nitrilase was down-regulated in both Fs and Cd.
Conclusion: The transcriptome analyses suggested radicicol resistance pathways, and deletions of the transporter genes indicated that they contribute to radicicol resistance.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (49)
참고문헌 신청
Liu CX / 1992 / Recent advances on ginseng research in China / J Ethnopharmacol 36:27~38
Chung HS / 1975 / Studies on Cylindrocarpon destructans(Zins. )Scholten causing root rot of ginseng / Rep Tottori Mycol Inst 12:127~138
강윤희 / 2014 / Development of a Selective Medium for the Fungal Pathogen Cylindrocarpon destructans Using Radicicol / The Plant Pathology Journal 30(4):432~436
Yun TK. / 2001 / Brief introduction of Panax ginseng C.A. Meyer / J Korean Med Sci. 16:S3~S5
Park JW / 2017 / A novel molecular diagnostic tool for detection of Ilyonectria radicicola, causal agent of root rot disease of ginseng / J Med Act Plants 5:20~27
Chung HS / 1975 / Studies on Cylindrocarpon destructans(Zins. )Scholten causing root rot of ginseng / Rep Tottori Mycol Inst 12:127~138
강윤희 / 2014 / Development of a Selective Medium for the Fungal Pathogen Cylindrocarpon destructans Using Radicicol / The Plant Pathology Journal 30(4):432~436
Yun TK. / 2001 / Brief introduction of Panax ginseng C.A. Meyer / J Korean Med Sci. 16:S3~S5
Park JW / 2017 / A novel molecular diagnostic tool for detection of Ilyonectria radicicola, causal agent of root rot disease of ginseng / J Med Act Plants 5:20~27
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UCI(KEPA) : I410-ECN-0101-2020-524-000504546