지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.3
- 수록면
- 44 - 54 (11page)
이용수
초록· 키워드
오류제보하기
Sexually transmitted infections (STIs) are caused by the spread of pathogens via sexual activity and can cause serious complications if left untreated, regardless of their symptoms. Therefore, early diagnosis of STI is important, and molecular diagnostic methods for rapid detection and monitoring are needed. In this study, we evaluated a multiplex polymerase chain reaction (PCR) kit for simultaneously detecting 13 different bacterial, fungal, and viral microorganisms that cause STIs. The kit performance was evaluated for its sensitivity, lot-to-lot variation, and interference in detecting different pathogens. Additionally, its clinical usefulness was evaluated by estimating its sensitivity and specificity for clinical samples. The limit of detection (LOD) was 0.021–50.104 copies for each pathogen. In the tests of lot-to-lot, 100% of positive samples were detected at low concentrations and negative samples all showed negative results. This result confirms that there is no the variation of lot-to-lot. In the test for interference between pathogens, the efficiency of amplification for each pathogen was not significantly reduced and no nonspecific amplification product was formed. We tested 322 vaginal swab samples using the multiplex PCR kit and confirmed that its clinical sensitivity and specificity were 100% for all pathogens. This multiplex PCR kit can be used widely for rapid diagnosis and monitoring of STIs.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Epidemiological Trends of Sexually Transmitted Infections Among Women in Cheonan, South Korea, 2011–2017
한국미생물·생명공학회지
2018 .01
-
서울 소재 일개 수탁 검사 기관에서 성병 검사의 실태조사: 다발성 성매개 감염병 중심으로
대한임상검사과학회지
2020 .01
-
Development of a Multiplex PCR for Rapid and Simultaneous Detection of Staphylococcus Species
한국미생물학회 학술대회논문집
2022 .10
-
Diagnosis of Viral Infection Using Real-time Polymerase Chain Reaction
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2018 .03
-
법과학 현장시료에서 Yfiler® PLUS PCR Amplification Kit의 민감도 연구
분석과학
2016 .02
-
Rapid identification of SARS-CoV-2 in the point-of-care using digital PCR-based Dr. PCR™ Di20K COVID-19 Detection Kit without viral RNA extraction
Genes & Genomics
2022 .05
-
Diagnostic evaluation of qRT-PCR-based kit and dPCR-based kit for COVID-19
Genes & Genomics
2021 .11
-
3Duplex dPCR System for Rapid Identification of Gram-Negative Pathogens in the Blood of Patients with Bloodstream Infection: A Culture- Independent Approach
Journal of Microbiology and Biotechnology
2021 .11
-
Simultaneous Detection of Four Foodborne Viruses in Food Samples Using a One-Step Multiplex Reverse Transcription PCR
Journal of Microbiology and Biotechnology
2018 .01
-
Occurrence and Multiplex PCR Detection of Citrus Yellow Vein Clearing Virus in Korea
The Plant Pathology Journal
2024 .04
-
Development of Multiplex RT-PCR for Simultaneous Detection of Garlic Viruses and the Incidence of Garlic Viral Disease in Garlic Genetic Resources
The Plant Pathology Journal
2015 .03
-
Comparative Analysis of Detection Methods for Food-borne Pathogens in Fresh-cut Agricultural Materials
생명과학회지
2021 .01
-
Resolution of Temporal-Multiplexing and Spatial-Multiplexing Stereoscopic Televisions
Current Optics and Photonics
2017 .02
-
Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti
The Plant Pathology Journal
2016 .02
-
Efficient Utilization of Korean Medical Fungal Pathogen Resource Bank for Clinical Research
대한의진균학회지
2019 .01
-
Analysis of Pathogens Causing Respiratory Infections in Sputum Samples
한국미생물학회 학술대회논문집
2015 .04
-
A New Multiplex-PCR for Urinary Tract Pathogen Detection Using Primer Design Based on an Evolutionary Computation Method
Journal of Microbiology and Biotechnology
2015 .01
-
Distribution Analysis of Candida albicans according to Sex and Age in Clinical Specimen Testing for Sexually Transmitted Diseases
Journal of Microbiology and Biotechnology
2023 .01
-
Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus
The Plant Pathology Journal
2016 .10
-
Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants
Research in Plant Disease(식물병연구)
2018 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0