지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.12
- 수록면
- 162 - 175 (14page)
이용수
초록· 키워드
오류제보하기
Purpose: Dysbiosis of gut microbiota has been reported to participate in the pathogenesis of colorectal cancer, but changes in microbiota due to radiotherapy have not been studied. In this study, we tried to elucidate the changes in the microbiome in rectal cancer after chemoradiotherapy using RNA sequencing analysis.
Materials and methods: We included 11 pairs of human rectal cancer tissues before and after irradiation between August 2016 and December 2017 and performed RNA sequencing analysis. Mapped reads to human reference genomes were used for pair-wise transcriptome comparisons, and unmapped (non-human) reads were then mapped to bacterial marker genes using PathSeq.
Results: At microbiome level, interindividual variability of mucosal microbiota was greater than the change in microbial composition during radiotherapy. This indicates that rapid homeostatic recovery of the mucosal microbial composition takes place short after radiotherapy. At single microbe level, Prevotella and Fusobacterium, which were identified as important causative microbes of the initiation and progression of rectal cancer were decreased by radiotherapy. Moreover, changes in Prevotella were associated with changes in the human transcriptome of rectal cancer. We also found that there was a gene cluster that increased and decreased in association with changes in microbial composition by chemoradiation.
Conclusion: This study revealed changes in tumor-associated microbial community by irradiation in rectal cancer. These findings can be used to develop a new treatment strategy of neoadjuvant therapy for locally advanced rectal cancer by overcoming radio-resistance or facilitating radio-sensitivity.
Materials and methods: We included 11 pairs of human rectal cancer tissues before and after irradiation between August 2016 and December 2017 and performed RNA sequencing analysis. Mapped reads to human reference genomes were used for pair-wise transcriptome comparisons, and unmapped (non-human) reads were then mapped to bacterial marker genes using PathSeq.
Results: At microbiome level, interindividual variability of mucosal microbiota was greater than the change in microbial composition during radiotherapy. This indicates that rapid homeostatic recovery of the mucosal microbial composition takes place short after radiotherapy. At single microbe level, Prevotella and Fusobacterium, which were identified as important causative microbes of the initiation and progression of rectal cancer were decreased by radiotherapy. Moreover, changes in Prevotella were associated with changes in the human transcriptome of rectal cancer. We also found that there was a gene cluster that increased and decreased in association with changes in microbial composition by chemoradiation.
Conclusion: This study revealed changes in tumor-associated microbial community by irradiation in rectal cancer. These findings can be used to develop a new treatment strategy of neoadjuvant therapy for locally advanced rectal cancer by overcoming radio-resistance or facilitating radio-sensitivity.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Metastasis associated genomic aberrations in stage II rectal cancer
Genes & Genomics
2016 .01
-
Treatment Planning Volume According to Change in Bladder Volume in Therapy of Proton Beam in Magnetic Field
Journal of Magnetics
2017 .12
-
Molecular fossils “pseudogenes” as functional signature in biological system
Genes & Genomics
2020 .01
-
LC-MS/MS-based Proteomic Analysis of Locally Advanced Rectal Tumors to Identify Biomarkers for Predicting Tumor Response to Neoadjuvant Chemoradiotherapy
Mass Spectrometry Letters
2022 .09
-
Lung Cancer might be Associated with Microbial Composition
한국미생물학회 학술대회논문집
2021 .08
-
Rare minisatellite alleles of MUC2-MS8 influence susceptibility to rectal carcinoma
Genes & Genomics
2021 .12
-
HPLC와 중수소 충돌기체 ICP/MS를 이용하여 분석한 셀레노단백질과 한국인 직장암과의 상관관계 연구
대한화학회지
2019 .12
-
Association between Long-term Exposure to Particulate Matter and Cause-specific Cancer Incidence Using Cancer Screenee Cohort of the National Cancer Center in South Korea
한국대기환경학회 학술대회논문집
2023 .10
-
The inhibitory effect of gut microbiota and its metabolites on colorectal cancer
Journal of Microbiology and Biotechnology
2020 .01
-
The Analysis of Human Microbiome as a Cause of Breast Cancer at a Young Age
한국미생물학회 학술대회논문집
2022 .10
-
The Role of Gut Microbiota in Modulating Tumor Growth and Anticancer Agent Efficacy
Molecules and Cells
2021 .01
-
The Microbiome-Immune Axis Therapeutic Effects in Cancer Treatments
Journal of Microbiology and Biotechnology
2022 .09
-
Anti-cancer Role of Probiotic-derived P8 Protein in Colorectal Cancer
한국미생물학회 학술대회논문집
2022 .10
-
The association of long non-coding RNA in the prognosis of oral squamous cell carcinoma
Genes & Genomics
2022 .03
-
Microbiota of Breast Tissue and Its Potential Association with Regional Recurrence of Breast Cancer in Korean Women
Journal of Microbiology and Biotechnology
2021 .12
-
The role of RNAi technique in cancer therapy
한국진공학회 학술발표회초록집
2017 .08
-
Roads to Construct and Re-build Plant Microbiota Community
The Plant Pathology Journal
2022 .10
-
Ovarian Cancer Prognostic Prediction Model Using RNA Sequencing Data
Genomics & informatics
2018 .01
-
Human Reference Gut Microbiome to Explore Human Microbial Dark Matter
한국미생물학회 학술대회논문집
2022 .10
-
Path to Bacterial Cancer Therapy
한국미생물학회 학술대회논문집
2020 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0