Stem cell analysis with intraductally treated mammary epithelial cells

Ah Young Yoon; Yun Young Kim; Joon-Hyop Lee; Yoo Seung Chung; Sangtae Choi; Jin Mo Kang; Heung Kyu Park; Yong Soon Chun

추천

검색

질문

자료유형: 학술저널

저자정보: Ah Young Yoon (Gachon University Gil Medical Center) Yun Young Kim (Gachon University Gil Medical Center) Joon-Hyop Lee (Gachon University Gil Medical Center) Yoo Seung Chung (Gachon University Gil Medical Center) Sangtae Choi (Gachon University Gil Medical Center) Jin Mo Kang (Gachon University Gil Medical Center) Heung Kyu Park (Gachon University Gil Medical Center) Yong Soon Chun (Gachon University Gil Medical Center)

저널정보: 대한종양외과학회 KOREAN JOURNAL OF CLINICAL ONCOLOGY Korean Journal of Clinical Oncology 제15권 제1호

발행연도: 2019.6

수록면: 27 - 33 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Purpose: The activity of mammary stem cells (MaSCs) is essential to mammary growth, differentiation and regeneration in cycles of pregnancy, lactation, and involution. The capability to recruit the mammary gland through the cycles is attributed to stem cells. It was shown that the intraductal (i.duc) injection of pegylated liposomal doxorubicin (PLD) to multiparous FVB/N mice was associated with a significantly reduced outgrowth potential of mammary gland cells. We have explored i.duc PLD’s effect on stem cell number and function in mouse mammary gland and aldehyde dehydrogenase (ALDH)’s availability as a mouse MaSC marker.
Methods: The total mammary epithelium was purified from 6 to 8-month-old FVB/N control and i.duc PLD-administered mice treated twice and analyzed by flow cytometry and limiting dilution cleared mammary fat pad transplants.
Results: There was no significant difference in the proportions of stem cell-enriched population (CD49fhighCD24med) between control and i.duc PLD-treated groups. However, we found a significant reduction in the outgrowth potential of CD49fhighCD24med and CD49fhighCD24medALDH(+) cells from i.duc PLD-treated mammary glands. We discovered that adding ALDH to CD49fhighCD24med had the possibility of better marker selection for MaSC of mice.
Conclusion: We present i.duc administration of PLD to reduce MaSC function, but not the number; and ALDH activity may add further selection of MaSCs to CD49f CD24 in mouse mammary glands. Screening of chemotherapeutic drugs or other natural products by this method of stem cell analysis may provide safe i.duc treatment in breast cancer.

#Intraductal #Mammary stem cell #Aldehyde dehydrogenase #CD49f #CD24

참고문헌 (19)

참고문헌 신청

Murata S / 2006 / Ductal access for prevention and therapy of mammary tumors / Cancer Res 66:638~645

Stearns V / 2011 / Preclinical and clinical evaluation of intraductally administered agents in early breast cancer / Sci Transl Med 3:106ra108

Chun YS / 2012 / Intraductal administration of a polymeric nanoparticle formulation of curcumin (NanoCurc) significantly attenuates incidence of mammary tumors in a rodent chemical carcinogenesis model:Implications for breast cancer chemoprevention in at-risk populations / Carcinogenesis 33:2242~2249

Chun YS / 2012 / Intraductally administered pegylated liposomal doxorubicin reduces mammary stem cell function in the mammary gland but in the long term, induces malignant tumors / Breast Cancer Res Treat 135:201~208

Stingl J / 2006 / Purification and unique properties of mammary epithelial stem cells / Nature 439:993~997

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!