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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Soybean cultivars with genetically low levels of stachyose enhance the utilization of soybean in food as well as feed uses. The objective of this research is to obtain the information on indirection selection of soybean lines with low stachyose content using DNA marker based on RS2 (rs2) gene. Two genetic populations were developed from the crosses of three parents (116-13 parent : low stachyose content, PI417227 and PI506903 parents: normal stachyose content). Twenty F2 plants of RS2_ genotype and twenty F2 plants of rs2rs2 genotype from each populations were harvested. Content of stachyose was detected by HPLC. Stachyose contents (g/kg) of 116-13, PI417227, PI506903 parents were 3.7, 23.7, and 17.8, respectively. In population 1, stachyose content 20 F2 plants with RS2_ genotype was 14.8 – 24.1 and stachyose content 20 F2 plants with rs2rs2 genotype was 2.1 – 4.7. In population 2, stachyose content 20 F2 plants with RS2_ genotype was 12.4 – 19.7 and stachyose content 20 F2 plants with rs2rs2 genotype was 2.1 – 5.0. Mean difference between RS2_ genotype and rs2rs2 genotype in population 1 and 2 was highly significant. From this results, selection of genetic lines with low stachyose content by DNA marker based on RS2 (rs2) gene will be possible.
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참고문헌 (17)
참고문헌 신청
Dierking EC / 2008 / Association of a soybean raffinose synthase gene with low raffinose and stachyose seed Phenotype / The Plant Genome 1 : 135 ~ 145
Hou A / 2009 / Genetic variability of seed sugar content in worldwide soybean germplasm collections / Crop Sci 49 : 903 ~ 912
Hymowitz T / 1972 / Relationship between the content of oil, protein, and sugar in soybean seed / Agron J 64 : 613 ~ 616
Hymowitz T / 1972 / Variability of sugar content in seed of Glycine max (L.) Merrill and G. soja Sieb and Zucc / Agron J 66 : 239 ~ 240
Jason DN / 2005 / Agronomic and seed characteristics of soybean with reduced raffinose and stachyose / Crop Sci 45 : 589 ~ 592
Hou A / 2009 / Genetic variability of seed sugar content in worldwide soybean germplasm collections / Crop Sci 49 : 903 ~ 912
Hymowitz T / 1972 / Relationship between the content of oil, protein, and sugar in soybean seed / Agron J 64 : 613 ~ 616
Hymowitz T / 1972 / Variability of sugar content in seed of Glycine max (L.) Merrill and G. soja Sieb and Zucc / Agron J 66 : 239 ~ 240
Jason DN / 2005 / Agronomic and seed characteristics of soybean with reduced raffinose and stachyose / Crop Sci 45 : 589 ~ 592
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