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Human mesenchymal stem cells (hMSC) are multipotent stromal cells that have great potential to differentiate into a variety of cell types such as osteocytes, chondrocytes, and myocytes. Although there have been many studies on their clinical availability, little is known about how intracellular signals can be modulated by topographic features of the extracellular matrix (ECM). In this study, we investigated whether and how microwavy-patterned extracellular matrix (ECM) could affect the signaling activity of focal adhesion kinase (FAK), a key cellular adhesion protein. The fluorescence resonance energy transfer (FRET)-based FAK biosensor-transfected cells are incubated on microwavy-patterned surfaces and then platelet derived growth factor (PDGF) are treated to trigger FAK signals, followed by monitoring through live-cell FRET imaging in real time. As a result, we report that PDGFinduced FAK was highly activated in cells cultured on microwavy-patterned surface with L or M type, while inhibited by H type-patterned surface. In further studies, PDGF-induced FAK signals are regulated by functional support of actin filaments, microtubules, myosin-related proteins, suggesting that PDGF-induced FAK signals in hMSC upon microwavy surfaces are dependent on cytoskeleton (CSK)-actomyosin networks. Thus, our findings not only provide new insight on molecular mechanisms on how FAK signals can be regulated by distinct topographical cues of the ECM, but also may offer advantages in potential applications for regenerative medicine and tissue engineering.
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참고문헌 (17)
참고문헌 신청
F. Rosso / 2004 / From cell-ECM interactions to tissue engineering / J. Cell. Physiol 199(2):174~180
S. Martino / 2012 / Stem cell-biomaterial interactions for regenerative medicine / Biotechnol. Adv 30(1):338~351
N. Joyce / 2010 / Mesenchymal stem cells for the treatment of neurodegenerative disease / Regen. Med 5(6):933~946
L. C. Lee / 2017 / Nanotopography controls cell cycle changes involved with skeletal stem cell self-renewal and multipotency / Biomaterials 116:10~20
W. Qian / 2017 / Nanotopographic regulation of human mesenchymal stem cell osteogenesis / ACS Appl. Mater. Interfaces 9(48):41794~41806
S. Martino / 2012 / Stem cell-biomaterial interactions for regenerative medicine / Biotechnol. Adv 30(1):338~351
N. Joyce / 2010 / Mesenchymal stem cells for the treatment of neurodegenerative disease / Regen. Med 5(6):933~946
L. C. Lee / 2017 / Nanotopography controls cell cycle changes involved with skeletal stem cell self-renewal and multipotency / Biomaterials 116:10~20
W. Qian / 2017 / Nanotopographic regulation of human mesenchymal stem cell osteogenesis / ACS Appl. Mater. Interfaces 9(48):41794~41806
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