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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제7호
- 발행연도
- 2019.1
- 수록면
- 1,033 - 1,042 (10page)
이용수
초록· 키워드
오류제보하기
Bacillus velezensis BS2 was isolated from meongge (common sea squirt) jeotgal, a Korean fermented seafood, and produces a bacteriocin, BacBS2, which strongly inhibits Listeria monocytogenes and Bacillus cereus. BacBS2 was partially purified by Q-Sepharose column chromatography after ammonium sulfate precipitation of the culture supernatant, then further purified by Sephadex G-50 column chromatography. Partially purified BacBS2 was estimated to be 6.5 kDa in size by Tricine-SDS PAGE and activity detection by gel-overlay. Enzyme treatment and FT-IR spectrum of partially purified BacBS2 confirmed its proteinaceous nature. BacBS2 was fully stable at pH 4-9, and half of activity was retained at pH 1-3. Full activity was retained after exposure to 80ºC for 15 min, but half of the activity was retained upon exposure to 90ºC for 15 min or 100ºC for 10 min. BacBS2 inhibited L. monocytogenes by bactericidal mode of action. B. velezensis BS2 and its BacBS2 seem useful as biopreservatives for fermented foods such as jeotgal.
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등록된 정보가 없습니다.
참고문헌 (34)
참고문헌 신청
Sumi CD / 2015 / Antimicrobial peptides of the genus Bacillus : a new era for antibiotics / Can. J. Microbiol 61:93~103
Stein T / 2005 / Bacillus subtilis antibiotics : structures, syntheses and specific functions / Mol. Microbiol 56:845~857
Leite JA / 2016 / Bacteriocin-like inhibitory substances(BLIS)produced by Bacillus cereus : preliminary characterization and application of partially purified extract containing BLIS for inhibiting Listeria monocytogenes in pineapple pulp / LWT-Food Sci. Technol 72:261~266
Abdel-Mohsein H / 2010 / Isolation of bacteriocin-like substances producing bacteria from finished cattle-manure compost and activity evaluation against some food-borne pathogenic and spoilage bacteria / J. Gen. Appl. Microbiol 56:151~161
Chehimi S / 2007 / Purification and partial amino acid sequence of thuricin S, a new anti-Listeria bacteriocin from Bacillus thuringiensis / Can. J. Microbiol 53:284~290
Stein T / 2005 / Bacillus subtilis antibiotics : structures, syntheses and specific functions / Mol. Microbiol 56:845~857
Leite JA / 2016 / Bacteriocin-like inhibitory substances(BLIS)produced by Bacillus cereus : preliminary characterization and application of partially purified extract containing BLIS for inhibiting Listeria monocytogenes in pineapple pulp / LWT-Food Sci. Technol 72:261~266
Abdel-Mohsein H / 2010 / Isolation of bacteriocin-like substances producing bacteria from finished cattle-manure compost and activity evaluation against some food-borne pathogenic and spoilage bacteria / J. Gen. Appl. Microbiol 56:151~161
Chehimi S / 2007 / Purification and partial amino acid sequence of thuricin S, a new anti-Listeria bacteriocin from Bacillus thuringiensis / Can. J. Microbiol 53:284~290
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