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논문 기본 정보
- 자료유형
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- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제5호
- 발행연도
- 2019.1
- 수록면
- 765 - 775 (11page)
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A new α-amylase-encoding gene (amySL3) of glycoside hydrolase (GH) family 13 was identified in soda lake isolate Alkalibacterium sp. SL3. The deduced AmySL3 shares high identities (82–98%) with putative α-amylases from the genus Alkalibacterium, but has low identities (<53%) with functionally characterized counterparts. amySL3 was successfully expressed in Escherichia coli, and the recombinant enzyme (rAmySL3) was purified to electrophoretic homogeneity. The optimal temperature and pH of the activity of the purified rAmySL3 were determined to be 45°C and pH 7.5, respectively. rAmySL3 was found to be extremely halophilic, showing maximal enzyme activity at a nearly saturated concentration of NaCl. Its thermostability was greatly enhanced in the presence of 4 M NaCl, and it was highly stable in 5 M NaCl. Moreover, the enzyme did not require calcium ions for activity, and was strongly resistant to a range of surfactants and hydrophobic organic solvents. The major hydrolysis products of rAmySL3 from soluble starch were maltobiose and maltotriose. The high ratio of acidic amino acids and highly negative electrostatic potential surface might account for the halophilic nature of AmySL3. The extremely halophilic, calcium-independent, and surfactant-resistant properties make AmySL3 a promising candidate enzyme for both basic research and industrial applications.
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참고문헌 (43)
참고문헌 신청
Guzman-Maldonado H / 1995 / Amylolytic enzymes and products derived from starch : a review / Crit. Rev. Food Sci. Nutr 35:373~403
van der Maarel MJ / 2002 / Properties and applications of starch-converting enzymes of the alpha-amylase family / J. Biotechnol 94:137~155
MacGregor EA / 1988 / Alpha-amylase structure and activity / J. Protein Chem 7:399~415
Janecek S / 2014 / Alpha-Amylase : an enzyme specificity found in various families of glycoside hydrolases / Cell Mol. Life Sci 71:1149~1170
Mikawlrawng K / 2016 / New and Future Developments in Microbial Biotechnology and Bioengineering / Elsevier :229~242
van der Maarel MJ / 2002 / Properties and applications of starch-converting enzymes of the alpha-amylase family / J. Biotechnol 94:137~155
MacGregor EA / 1988 / Alpha-amylase structure and activity / J. Protein Chem 7:399~415
Janecek S / 2014 / Alpha-Amylase : an enzyme specificity found in various families of glycoside hydrolases / Cell Mol. Life Sci 71:1149~1170
Mikawlrawng K / 2016 / New and Future Developments in Microbial Biotechnology and Bioengineering / Elsevier :229~242
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