지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제1호
- 발행연도
- 2019.1
- 수록면
- 127 - 140 (14page)
이용수
초록· 키워드
오류제보하기
Since 1990, many nucleic acid expression platforms consisting of DNA or RNA have been developed. However, although RNA expression platforms have been relatively neglected, several such platforms capped at the 5’ end of RNA by an anti-reverse cap analog have now been developed. At the same time, the capping reaction is a bottleneck in the production of such platforms, with high cost and low efficiency. Here, we investigated several viral and eukaryotic internal ribosome entry sites (IRESs) to develop an optimal RNA expression platform, because IRES-dependent translation does not require a capping step. RNA expression platforms constructed with IRESs from the 5’ untranslated regions of the encephalomyocarditis virus (EMCV) and the intergenic region of the cricket paralysis virus (CrPV) showed sufficient expression efficiency compared with cap-dependent RNA expression platforms. However, eukaryotic IRESs exhibited a lower viral IRES expression efficiency. Interestingly, the addition of a poly(A) sequence to the 5’ end of the coxsackievirus B3 (CVB3) IRES (pMA-CVB3) increased the expression level compared with the CVB3 IRES without poly(A) (pCVB3). Therefore, we developed two multiexpression platforms (termed pMA-CVB3-EMCV and pCrPV-EMCV) by combining the IRESs of CVB3, CrPV, and EMCV in a single-RNA backbone. The pMA-CVB3-EMCV-derived RNA platform showed the highest expression level. Moreover, it clearly exhibited expression in mouse muscles in vivo. These RNA expression platforms prepared using viral IRESs will be useful in developing potential RNA-based prophylactic or therapeutic vaccines, because they have better expression efficiency and do not need a capping step.
목차
등록된 정보가 없습니다.
참고문헌 (50)
참고문헌 신청
Wolff JA / 1990 / Direct gene transfer into mouse muscle in vivo / Science 247:1465~1468
Marć MA / 2015 / Nucleic acid vaccination strategies against infectious diseases / Expert. Opin. Drug Deliv 12:1851~1865
Sahin U / 2014 / mRNA-based therapeutics—developing a new class of drugs / Nat. Rev. Drug Discov 13:759~780
Kallen KJ / 2014 / A development that may evolve into a revolution in medicine : mRNA as the basis for novel, nucleotide-based vaccines and drugs / Ther. Adv. Vaccines 2:10~31
Probst J / 2012 / Gene vaccines / Springer Verlag :223~245
Marć MA / 2015 / Nucleic acid vaccination strategies against infectious diseases / Expert. Opin. Drug Deliv 12:1851~1865
Sahin U / 2014 / mRNA-based therapeutics—developing a new class of drugs / Nat. Rev. Drug Discov 13:759~780
Kallen KJ / 2014 / A development that may evolve into a revolution in medicine : mRNA as the basis for novel, nucleotide-based vaccines and drugs / Ther. Adv. Vaccines 2:10~31
Probst J / 2012 / Gene vaccines / Springer Verlag :223~245
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
The Characteristics of RNA Vaccine
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2016 .09
-
Real-Time Temporal Dynamics of Bicistronic Expression Mediated by Internal Ribosome Entry Site and 2A Cleaving Sequence
Molecules and Cells
2019 .01
-
Circular RNAs in and out of Cells: Therapeutic Usages of Circular RNAs
Molecules and Cells
2023 .01
-
Ovarian transcriptome profiles associated with sexual maturation in Pacific abalone (Haliotis discus hannai)
Genes & Genomics
2020 .01
-
Comparative analysis of the Down syndrome hippocampal non-coding RNA transcriptomes using a mouse model
Genes & Genomics
2020 .01
-
Determinants of RNA Function
한국미생물학회 학술대회논문집
2022 .10
-
Combining the Power of Advanced Proteome-wide Sample Preparation Methods and Mass Spectrometry for defining the RNA-Protein Interactions
Mass Spectrometry Letters
2022 .12
-
Characterization and functional analysis of Krtap11-1 during hair follicle development in Angora rabbits (Oryctolagus cuniculus)
Genes & Genomics
2020 .01
-
How are Bayesian and Non-Parametric Methods Doing a Great Job in RNA-Seq Differential Expression Analysis?: A Review
CSAM(Communications for Statistical Applications and Methods)
2015 .03
-
Heterogeneous Sequences of Brain Cytoplasmic 200 RNA Formed by Multiple Adenine Nucleotide Insertions
Molecules and Cells
2019 .01
-
Incredible RNA: Dual Functions of Coding and Noncoding
Molecules and Cells
2016 .05
-
Cholic Acid Attenuates ER Stress-Induced Cell Death in Coxsackievirus- B3 Infection
Journal of Microbiology and Biotechnology
2018 .01
-
Expression Analysis of Long non-coding RNA-NORAD in Breast Cancerous Tissues
한국분석과학회 학술대회
2022 .11
-
Stress Granules Inhibit Coxsackievirus B3-Mediated Cell Death via Reduction of Mitochondrial Reactive Oxygen Species and Viral Extracellular Release
Journal of Microbiology and Biotechnology
2023 .05
-
Secondary Structure for RNA Aptamers Binding to Guanine-Rich Sequence in the 5’-UTR RNA of N-Ras Oncogene
대한화학회지
2021 .04
-
Application of small RNA for diagnosis and prognosis
한국자기학회 학술연구발표회 논문개요집
2024 .05
-
Scutellaria baicalensis Inhibits Coxsackievirus B3-Induced Myocarditis Via AKT and p38 Pathways
Journal of Microbiology and Biotechnology
2019 .01
-
High Resolution Mapping of RNA-Protein Interactions & SARS-CoV-2 RNA interactome
한국분석과학회 학술대회
2021 .05
-
Analysis of Whole Transcriptome Sequencing Data: Workflow and Software
Genomics & informatics
2015 .01
-
Comparison of Digital PCR and Quantitative PCR with Various SARS-CoV-2 Primer-Probe Sets
Journal of Microbiology and Biotechnology
2021 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0