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- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.5
- 수록면
- 42 - 42 (1page)
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Mass spectrometry (MS) based proteomics has been used to elucidate the RNA-interactome in various biological systems. However, the profiling resolution of RNA-binding sites or domains still remains in poor and vague stage. Here, I will present a couple of recently developed methods for high-resolution mapping of RNA-binding sites or regions using chemical tools; 1) RBS-ID and 2) FAX-RIC. RNA-binding site (RBS)-ID is a method that uses hydrofluoric acid to fully cleave RNA into mono-nucleosides, thereby minimizing the search space to drastically enhance coverage and to reach single amino acid resolution. The simple mono-nucleoside adducts offer a confident and quantitative measure of direct RNA–protein interaction. Using RB ... 전체 초록 보기
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