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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제5호
- 발행연도
- 2019.1
- 수록면
- 785 - 793 (9page)
이용수
초록· 키워드
오류제보하기
Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is the most damaging disease in Brassica crops around the world. In this study, we developed a molecular marker specific to Xcc race 5. To do this, the available whole genome sequences of Xcc races/strains and Xc subspecies were aligned and identified a highly variable genomic region (XccR5-89.2). Subsequently, a primer set covering the ‘XccR5-89.2’ region was designed and tested against the genomic DNA of Xcc races/strains, Xc subspecies and other plant-infecting bacterial strains (Pseudomonas syringae pv. maculicola and Erwinia carotovora subsp. carotovora). The results showed that the ‘XccR5-89.2’ primer pair amplified a 2,172-bp fragment specific to Xcc race 5. Moreover, they also amplified a 1,515-bp fragment for Xcc race 1 and an over 3,000-bp fragment for Xcc race 3. However, they did not amplify any fragments from the remaining Xcc races/strains, subspecies or other bacterial strains. The ‘XccR5-89.2’ primer pair was further PCR amplified from race-unknown Xcc strains and ICMP8 was identified as race 5 among nine race-unknown Xcc strains. Further cloning and sequencing of the bands amplified from race 5 and ICMP8 with ‘XccR5-89.2’ primers revealed both carrying identical sequences. The results showed that the ‘XccR5-89.2’ marker can effectively and proficiently detect, and identify Xcc race 5 from Xcc races/strains, subspecies and other plant-infecting bacteria. To our knowledge, this is the first report for an Xcc race 5-specific molecular marker.
목차
등록된 정보가 없습니다.
참고문헌 (40)
참고문헌 신청
Vicente JG / 2001 / Identification and origin of Xanthomonas campestris pv, campestris races and related pathovars / Phytopathology 91:492~499
Soengas P / 2007 / Identification of quantitative trait loci for resistance to Xanthomonas campestris pv, campestris in Brassica rapa / Theor. Appl. Genet 114:637~645
Lema M / 2012 / Discrimination of Xanthomonas campestris pv, campestris races among strains from northwestern Spain by Brassica spp. genotypes and rep-PCR / Eur. J. Plant Pathol 133:159~169
Williams PH / 1980 / Black rot : a continuing threat to world crucifers / Plant Dis 64:736~742
Cook AA / 1952 / Studies on the disease cycle of black rot of crucifers / Phytopathology 42:162~167
Soengas P / 2007 / Identification of quantitative trait loci for resistance to Xanthomonas campestris pv, campestris in Brassica rapa / Theor. Appl. Genet 114:637~645
Lema M / 2012 / Discrimination of Xanthomonas campestris pv, campestris races among strains from northwestern Spain by Brassica spp. genotypes and rep-PCR / Eur. J. Plant Pathol 133:159~169
Williams PH / 1980 / Black rot : a continuing threat to world crucifers / Plant Dis 64:736~742
Cook AA / 1952 / Studies on the disease cycle of black rot of crucifers / Phytopathology 42:162~167
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