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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제8호
- 발행연도
- 2019.1
- 수록면
- 1,310 - 1,315 (6page)
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초록· 키워드
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Hyaluronidases enhance therapeutic drug transport by breaking down the hyaluronan barrier to lymphatic and capillary vessels, facilitating their tissue absorption. Commercially available hyaluronidases are bovine in origin; however, they pose risks such as bovine spongiform encephalopathy. The present study aimed to develop a novel, highly active hyaluronidase and assess its function. Therefore, in order to find the most efficient active hyaluronidase, we produced several shortened hyaluronidases with partial removal of the N- or C-terminal regions. Moreover, we created an enzyme that connected six histidines onto the end of the hyaluronidase C-terminus. This simplified subsequent purification using Ni2+ affinity chromatography, making it feasible to industrialize this highly active recombinant hyaluronidase which exhibited catalytic activity equal to that of the commercial enzyme. Therefore, this simple and effective isolation method could increase the availability of recombinant hyaluronidase for research and clinical purposes.
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참고문헌 (17)
참고문헌 신청
Zhu Y / 2019 / Hyaluronan in adipogenesis, adipose tissue physiology and systemic metabolism / Matrix Biol 78-79:284~291
Camaioni A / 1996 / Proteoglycans and proteins in the extracellular matrix of mouse cumulus cell-oocyte complexes / Arch. Biochem. Biophys 325:190~198
Viola M / 2015 / Regulated Hyaluronan Synthesis by Vascular Cells / Int. J. Cell Biol 2015:208303
Aya KL / 2014 / Hyaluronan in wound healing : rediscovering a major player / Wound Repair Regen 22:579~593
Nguyen N / 2017 / Human hyaluronic acid synthase-1 promotes malignant transformation via epithelial-to-mesenchymal transition, micronucleation and centrosome abnormalities / Cell Commun. Signal 15(1):48
Camaioni A / 1996 / Proteoglycans and proteins in the extracellular matrix of mouse cumulus cell-oocyte complexes / Arch. Biochem. Biophys 325:190~198
Viola M / 2015 / Regulated Hyaluronan Synthesis by Vascular Cells / Int. J. Cell Biol 2015:208303
Aya KL / 2014 / Hyaluronan in wound healing : rediscovering a major player / Wound Repair Regen 22:579~593
Nguyen N / 2017 / Human hyaluronic acid synthase-1 promotes malignant transformation via epithelial-to-mesenchymal transition, micronucleation and centrosome abnormalities / Cell Commun. Signal 15(1):48
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