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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 193 - 199 (7page)
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초록· 키워드
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The 2b protein of Cucumber mosaic virus (CMV) subgroup IB strain IA (IA2b) suppresses the sense transgene-induced posttranscriptional gene silencing (S-PTGS) but not the inverted repeat-induced post-transcriptional gene silencing (IR-PTGS) of a tobacco microsome-localized α-linolenate synthase gene (NtFAD3). In contrast, the 2b protein of CMV subgroup IB strain SD (SD2b) has been reported to suppress IR-PTGS. We overexpressed the SD2b gene in tobacco, and this transgenic line was crossed with the transgenic plants showing S-PTGS and IR-PTGS of the NtFAD3 gene. The phenotype of offspring showed that SD2b inhibited S-PTGS but not IR-PTGS. Next, we determined the suppressor activity of IA2b and SD2b proteins in a transient IR-PTGS assay. The transient expression of firefly luciferase (LUC) gene was efficiently decreased by IR-PTGS. Co-infiltration of SD2b and IA2b gene partially suppressed IR-PTGS of the LUC gene in the wild-type plants; however, these 2b proteins did not suppress IR-PTGS in the RNA-dependent RNA polymerase6 (RDR6) knockdown plants. It has been reported that RDR6-dependent secondary small interfering RNA synthesis does not occur in the IR-PTGS, when targeting endogenous genes such as NtFAD3, but is efficiently induced in the IR-PTGS of the reporter transgenes. These results indicate that the SD2b and IA2b preferentially suppress the RDR6-dependent silencing pathway but do not suppress the RDR6-independent IR-PTGS pathway.
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등록된 정보가 없습니다.
참고문헌 (42)
참고문헌 신청
Azevedo J / 2011 / Taking RISCs with Ago hookers / Curr Opin Plant Biol 14:594~600
Béclin C / 1998 / Infection of tobacco or Arabidopsis plants by CMV counteracts systemic post-transcriptional silencing of nonviral(trans)genes / Virology 252:313~317
Csorba T / 2015 / Viral silencing suppressors:tools forged to fine-tune host-pathogen coexistence / Virology 479?480:85~103
Dong K / 2016 / Two amino acids near the N-terminus of Cucumber mosaic virus 2b play critical roles in the suppression of RNA silencing and viral infectivity / Mol Plant Pathol 17:173~183
Du ZY / 2007 / 2b ORFs encoded by subgroup IB strains of cucumber mosaic virus induce differential virulence on Nicotiana species / J Gen Virol 88:2596~2604
Béclin C / 1998 / Infection of tobacco or Arabidopsis plants by CMV counteracts systemic post-transcriptional silencing of nonviral(trans)genes / Virology 252:313~317
Csorba T / 2015 / Viral silencing suppressors:tools forged to fine-tune host-pathogen coexistence / Virology 479?480:85~103
Dong K / 2016 / Two amino acids near the N-terminus of Cucumber mosaic virus 2b play critical roles in the suppression of RNA silencing and viral infectivity / Mol Plant Pathol 17:173~183
Du ZY / 2007 / 2b ORFs encoded by subgroup IB strains of cucumber mosaic virus induce differential virulence on Nicotiana species / J Gen Virol 88:2596~2604
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