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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.12
- 수록면
- 389 - 397 (9page)
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RNA silencing is a sequence-specific form of epigenetic regulation that targets invasive nucleic acids. RNA-dependent RNA polymerase6 (RDR6) converts target RNA molecules, such as transgene transcripts, into double-stranded RNAs (dsRNAs) during posttranscriptional gene silencing (PTGS). Then, these dsRNAs are processed into small RNAs that guide sequencespecific RNA degradation. T-DNA-derived small RNAs are generated during the transfer of T-DNA from Agrobacterium to plant cells and compromise the function of the genes in the T-DNA. In the present study, we produced selection-markerfree transgenic tobacco plants using the MAT vector system, and expression of the tobacco RDR6 gene (NtRDR6) was suppressed using inverted-repeat-induced PTGS. Reduced expression of the NtRDR6 gene improved the transient expression of the transgene in the agroinfiltrated leaves and enhanced the production of hairy roots after infection with Agrobacterium containing a root-inducing T-DNA. The expression level of the sense transgene was determined in individual hairy roots, and knockdown of the NtRDR6 gene did not affect the distribution of the expression levels in individual transformants. These results indicate that NtRDR6 partially inhibited T-DNA function during T-DNA transfer but did not affect the expression of the transgene in stable transformants, except in transformants showing sense-transgene-induced PTGS.
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참고문헌 (46)
참고문헌 신청
Baumberger N / 2005 / Arabidopsis ARGONAUTE1 is an RNA slicer that selectively recruits microRNAs and short interfering RNAs / Proc Natl Acad Sci USA 102:11928~11933
Bilichak A / 2014 / Transient down-regulation of the RNA silencing machinery increases efficiency of Agrobacterium-mediated transformation of Arabidopsis / Plant Biotechnol J 12:590~600
Boualem A / 2016 / The battle for survival between viruses and their host plants / Curr Opin Virol 17:32~38
Brodersen P / 2006 / The diversity of RNA silencing pathways in plants / Trends Genet 22:268~280
Chuang CF / 2000 / Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana / Proc Natl Acad Sci USA 97:4985~4990
Bilichak A / 2014 / Transient down-regulation of the RNA silencing machinery increases efficiency of Agrobacterium-mediated transformation of Arabidopsis / Plant Biotechnol J 12:590~600
Boualem A / 2016 / The battle for survival between viruses and their host plants / Curr Opin Virol 17:32~38
Brodersen P / 2006 / The diversity of RNA silencing pathways in plants / Trends Genet 22:268~280
Chuang CF / 2000 / Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana / Proc Natl Acad Sci USA 97:4985~4990
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