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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 211 - 218 (8page)
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초록· 키워드
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Feverfew (Tanacetum parthenium) is a medicinal herb that has multiple pharmacologic properties. This plant is rich parthenolide (PN). Hairy roots (HRs) culture is a valuable biotechnological tool for the production of plant secondary metabolites. Furthermore, application of elicitors is one of the most effective strategies to induce the metabolite biosynthesis. This study has investigated the effects of various elicitors on the production of PN in feverfew hairy root culture and the expression of parthenolide synthase (TpPTS) gene. Three elicitors were used separately and in combination, including yeast extract (YE) (2.5 mgL− 1), methyl jasmonate (MJ) (100 μM), Ag+ (100 μM), YE + MJ, Ag+ + MJ, YE + Ag+, and YE + MJ + Ag+ which were added to the 1/2MS medium on day 18 and after 48 h and 96 h, hairy roots were collected to determine the PN content. Total RNA was extracted from hairy roots and used for cDNA synthesis and real-time PCR analysis to investigate the gene expression of TpPTS. The PN content was determined by HPLC. The highest PN content (0.05 mg g− 1 dry weight) was achieved after application of elicitors (in 2.5 mgL− 1 YE and 100 μM MJ) and the highest expression of TpPTS gene also occurred after 96 h in the same treatment. It was concluded that some of the combined elicitors could efficiently increase the parthenolide content of feverfew in vitro culture. The additive effect of elicitors might be due to the separate mechanisms of each substance that is acting in an independent pathway.
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참고문헌 (46)
참고문헌 신청
Abraham F / 2011 / Effect of yeast extract and chitosan on shoot proliferation, morphology and antioxidant activity of Curcuma mangga in vitro plantlets / Afr J Biotechnol 10:7787~7795
Cai Z / 2012 / Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera / Plant Cell Tissue Organ 108:401~409
Cheng Q / 2013 / Effects of combined elicitors on tanshinone metabolic profiling and SmCPS expression in Salvia miltiorrhiza hairy root cultures / Molecules 18:7473~7485
De Kraker J-W / 2002 / Biosynthesis of costunolide, dihydrocostunolide, and leucodin. Demonstration of cytochrome P450-catalyzed formation of the lactone ring present in sesquiterpene lactones of chicory / Plant Physiol 129:257~268
Gupta SK / 2011 / Enhanced tanshinone production in hairy roots of ‘Salvia miltiorrhiza Bunge’under the influence of plant growth regulators in liquid culture / Bot Stud 52:435~443
Cai Z / 2012 / Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera / Plant Cell Tissue Organ 108:401~409
Cheng Q / 2013 / Effects of combined elicitors on tanshinone metabolic profiling and SmCPS expression in Salvia miltiorrhiza hairy root cultures / Molecules 18:7473~7485
De Kraker J-W / 2002 / Biosynthesis of costunolide, dihydrocostunolide, and leucodin. Demonstration of cytochrome P450-catalyzed formation of the lactone ring present in sesquiterpene lactones of chicory / Plant Physiol 129:257~268
Gupta SK / 2011 / Enhanced tanshinone production in hairy roots of ‘Salvia miltiorrhiza Bunge’under the influence of plant growth regulators in liquid culture / Bot Stud 52:435~443
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