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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 340 - 346 (7page)
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초록· 키워드
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In this study, the effect of elicitors such as yeast extract (YE), methyl jasmonate (MeJA) and salicylic acid (SA) on the accumulation of eurycomanone in Eurycoma longifolia cell cultures were investigated. Suspension cells of E. longifolia was cultured in Murashige and Skoog (MS) medium supplemented with 30 g/L sucrose, 1.25 mg/L naphthaleneacetic acid (NAA) and 1 mg/L kinetin at a shaking speed of 120 rpm. Elicitors were added in the culture at different concentrations and times to stimulate eurycomanone accumulation in the Eurycoma longifolia cells. Eurycomanone content was determined by HPLC with a C18 column, flow rate of 0.8 mL/min, run time of 17.5 min, and a detector wavelength of 254 nm. The stationary phase was silica gel and the mobile phase was acetonitrile: H2O. Non-elicited cells were used as the control. The study showed the effect of different elicitor concentrations, YE at 200 mg/L, MeJA at 20 μM and SA at 20 μM stimulated high production of eurycomanone. In which, treatment of 20 μM MeJA after 4 days of culture resulted in the highest accumulation of this compound (17.36 mg/g dry weight), approximately 10-fold higher than that of untreated cells (1.70 mg/g dry weight).
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참고문헌 (29)
참고문헌 신청
Abdullah N / 2016 / 9-methoxycanthin-6-one production in elicited hairy roots culture of E. longifolia / AIP Conf Proc 1784:020023-1~020023-6
Cai Z / 2014 / Chitosan or yeast extract enhance the accumulation of eight phenolic acids in cell suspension cultures of Malus × domestica Borkh / J Hortic Sci Biotechnol 89:93~99
Chan KL / 2004 / Antiplasmodial studies of Eurycoma longifolia Jack using the lactate dehydrogenase assay of Plasmodium falciparum / J Ethnopharmacol 92:223~227
Chee FM / 2015 / Effects of methyl-jasmonate on 9-methoxycanthin-6-one content in Eurycoma longifolia (tongkat ali) root culture / Pak J Bot 47:897~904
Coste A / 2011 / Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum / Plant Cell Tiss Organ Cult 106:279~288
Cai Z / 2014 / Chitosan or yeast extract enhance the accumulation of eight phenolic acids in cell suspension cultures of Malus × domestica Borkh / J Hortic Sci Biotechnol 89:93~99
Chan KL / 2004 / Antiplasmodial studies of Eurycoma longifolia Jack using the lactate dehydrogenase assay of Plasmodium falciparum / J Ethnopharmacol 92:223~227
Chee FM / 2015 / Effects of methyl-jasmonate on 9-methoxycanthin-6-one content in Eurycoma longifolia (tongkat ali) root culture / Pak J Bot 47:897~904
Coste A / 2011 / Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum / Plant Cell Tiss Organ Cult 106:279~288
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