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Xanthomonas oryzae pv. oryzae (Xoo) is a plant pathogen, which causes a bacterial blight of rice. The bacterial blightis one of the most devastating diseases of rice in most of the rice growing countries and there is no effective pesticideagainst bacterial blight. The β-ketoacyl-acyl carrier protein synthase III (FabH) plays a key role in fatty acid synthesis(FAS) and is a promising drug target for the development of antibacterial agents. Xoo0878 gene, a fabH gene, from Xoowas cloned and its gene product Xoo0878 was expressed, purified and crystallized. Xoo0878 crystal diffracted to 2.1Åresolution and belonged to the triclinic space group P1, with unit-cell parameters a = 57.3Å, b = 64.7Å, c = 104.2Å and α =81.6°, β = 84.7°, γ = 74.4°. There are four monomers in the asymmetric unit, with a corresponding crystal volume per proteinweight of 2.65 Å3 Da−1 and a solvent content of 53.6%. Xoo0878 structure will be useful to develop new antibacterial agentsagainst Xoo.
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참고문헌 (13)
참고문헌 신청
Choi, K. H. / 2000 / beta-ketoacyl-acyl carrier protein synthase III(FabH)is a determining factor in branched-chain fatty acid biosynthesis / J Bacteriol 182:365~370
Choi, K. H. / 2000 / Identification and substrate specificity of beta-ketoacyl(acyl carrier protein)synthase III(mtFabH)from Mycobacterium tuberculosis / J Biol Chem 275:28201~28207
Gago, G. / 2011 / Fatty acid biosynthesis in actinomycetes / FEMS Microbiol Rev 35:475~497
Gajiwala, K. S. / 2009 / Crystal structures of bacterial FabH suggest a molecular basis for the substrate specificity of the enzyme / FEBS Lett 583:2939~2946
Heath, R. J. / 1996 / Regulation of fatty acid elongation and initiation by acyl-acyl carrier protein in Escherichia coli / J Biol Chem 271:1833~1836
Choi, K. H. / 2000 / Identification and substrate specificity of beta-ketoacyl(acyl carrier protein)synthase III(mtFabH)from Mycobacterium tuberculosis / J Biol Chem 275:28201~28207
Gago, G. / 2011 / Fatty acid biosynthesis in actinomycetes / FEMS Microbiol Rev 35:475~497
Gajiwala, K. S. / 2009 / Crystal structures of bacterial FabH suggest a molecular basis for the substrate specificity of the enzyme / FEBS Lett 583:2939~2946
Heath, R. J. / 1996 / Regulation of fatty acid elongation and initiation by acyl-acyl carrier protein in Escherichia coli / J Biol Chem 271:1833~1836
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