지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.97 No.5
- 발행연도
- 2019.11
- 수록면
- 230 - 238 (9page)
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초록· 키워드
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Purpose: This study was aimed to investigate the combination effect of endoxifen and emodin on estrogen receptor (ER) positive breast cancer cell lines and to explain the mechanism of the combination effect.
Methods: We conducted this study on MCF-7 (ER+/human epidermal growth factor receptor-2 [HER2]-), T47D (ER+/ HER2-), ZR-75-1 (ER+/HER2+), and BT474 (ER+/HER2+) cell lines, which confirmed combination effect of endoxifen and emodin. Optimal concentrations for combination were determined to study the effects on proliferation of MCF-7 and ZR- 75-1 cells. Analysis of the combination effect was carried out in the CompuSyn software. The combination of downstream mechanisms, and combined effects of other similar compounds were tested on the MCF-7 and ZR 75-1 cell lines. Protein expression was confirmed by western blot.
Results: The combination of endoxifen and emodin had antagonistic effects on MCF-7 and ZR-75-1cell lines (combination index > 1). We validated the antagonistic effect in T47D and BT474 cell lines. During the combined treatment, the results showed elevated amounts of cyclin D1 and phosphorylated extracellular signal-regulated kinase (pERK). Analysis of drug interactions showed antagonistic effect between endoxifen and chemical compounds similar to emodin, such as chrysophanol or rhein, in MCF-7 and ZR-75-1 cells.
Conclusion: Addition of emodin attenuated tamoxifen’s treatment effect via cyclin D1 and pERK up-regulation in ER-positive breast cancer cell lines.
Methods: We conducted this study on MCF-7 (ER+/human epidermal growth factor receptor-2 [HER2]-), T47D (ER+/ HER2-), ZR-75-1 (ER+/HER2+), and BT474 (ER+/HER2+) cell lines, which confirmed combination effect of endoxifen and emodin. Optimal concentrations for combination were determined to study the effects on proliferation of MCF-7 and ZR- 75-1 cells. Analysis of the combination effect was carried out in the CompuSyn software. The combination of downstream mechanisms, and combined effects of other similar compounds were tested on the MCF-7 and ZR 75-1 cell lines. Protein expression was confirmed by western blot.
Results: The combination of endoxifen and emodin had antagonistic effects on MCF-7 and ZR-75-1cell lines (combination index > 1). We validated the antagonistic effect in T47D and BT474 cell lines. During the combined treatment, the results showed elevated amounts of cyclin D1 and phosphorylated extracellular signal-regulated kinase (pERK). Analysis of drug interactions showed antagonistic effect between endoxifen and chemical compounds similar to emodin, such as chrysophanol or rhein, in MCF-7 and ZR-75-1 cells.
Conclusion: Addition of emodin attenuated tamoxifen’s treatment effect via cyclin D1 and pERK up-regulation in ER-positive breast cancer cell lines.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (27)
참고문헌 신청
고범석 / 2012 / Changing Patterns in the Clinical Characteristics of Korean Breast Cancer from 1996-2010 Using an Online Nationwide Breast Cancer Database / Journal of Breast Cancer 15(4):393~400
Osborne CK / 1998 / Tamoxifen in the treatment of breast cancer / N Engl J Med 339:1609~1618
1998 / Tamoxifen for early breast cancer: an overview of the randomised trials. Early Breast Cancer Trialists’ Collaborative Group / Lancet 351:1451~1467
Burstein HJ / 2014 / Adjuvant endocrine therapy for women with hormone receptor-positive breast cancer : american society of clinical oncology clinical practice guideline focused update / J Clin Oncol 32:2255~2269
Aiello Bowles EJ / 2012 / Patientreported discontinuation of endocrine therapy and related adverse effects among women with early-stage breast cancer / J Oncol Pract 8:e149~e157
Osborne CK / 1998 / Tamoxifen in the treatment of breast cancer / N Engl J Med 339:1609~1618
1998 / Tamoxifen for early breast cancer: an overview of the randomised trials. Early Breast Cancer Trialists’ Collaborative Group / Lancet 351:1451~1467
Burstein HJ / 2014 / Adjuvant endocrine therapy for women with hormone receptor-positive breast cancer : american society of clinical oncology clinical practice guideline focused update / J Clin Oncol 32:2255~2269
Aiello Bowles EJ / 2012 / Patientreported discontinuation of endocrine therapy and related adverse effects among women with early-stage breast cancer / J Oncol Pract 8:e149~e157
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UCI(KEPA) : I410-ECN-0101-2019-514-001293912