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Aloe vera has been used in the pharmaceutical, food and cosmetic industry for its therapeutic properties. However, there are not many current studies on the microstructure of A. vera compared to studies on the chemical constituents and health efficacy of A. vera. Therefore, we compared the morphology of an A. vera leaf using an optical microscope, a conventional scanning electron microscope (SEM) and a cryo-SEM. Especially, this study focused on observing the gel in the inner leaf of A. vera, which is challenging using standard imaging techniques. We found that cryo-SEM is most suitable method for the observation of highly hydrated biomaterials such as A. vera without removing moisture in samples. In addition, we found the optimal analytical conditions of cryo-SEM. The sublimation conditions of –100°C and 10 minutes possibly enable the surface of the inner leaf of A. vera to be observed in their “near life-like” state with retaining moisture. The experiment was repeated with A. arborescens and A. saponaria to confirm the feasibility of the conditions. The results of this study can be applied towards the basic research of aloe and further extend previous knowledge about the surface structures of the various succulent plants.
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참고문헌 (26)
참고문헌 신청
Balaji A / 2015 / Biomaterials based nano-applications of Aloe vera and its perspective: a review / RSC Adv 5:86199~86213
Betzig E / 1991 / Breaking the diffraction barrier: optical microscopy on a nanometric scale / Science 251:1468~1470
Bozzola J J / 1999 / Electron Microscopy: Principles and Techniques for Biologists / Jones and Bartlett Learning
Braet F / 1997 / Drying cells for SEM, AFM and TEM by hexamethyldisilazane: a study on hepatic endothelial cells / J. Microsc 186:84~87
Choi S / 2003 / A review on the relationship between Aloevera components and their biologic effects / Semin. Integr. Med 1:53~62
Betzig E / 1991 / Breaking the diffraction barrier: optical microscopy on a nanometric scale / Science 251:1468~1470
Bozzola J J / 1999 / Electron Microscopy: Principles and Techniques for Biologists / Jones and Bartlett Learning
Braet F / 1997 / Drying cells for SEM, AFM and TEM by hexamethyldisilazane: a study on hepatic endothelial cells / J. Microsc 186:84~87
Choi S / 2003 / A review on the relationship between Aloevera components and their biologic effects / Semin. Integr. Med 1:53~62
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