지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Cryo-electron microscopy (cryo-EM) single particle analysis has become a mainstream structural biology tool andtherefore access to high-end transmission electron microscope (TEM) dedicated to high resolution cryo-EM is critical.
At Korea Basic Science Institute (KBSI), a facility dedicated to biological electron microscopy was established in 2016,and High Resolution Bio-TEM (HR Bio-TEM, Titan Krios, Thermo Fisher Scientific Inc., USA) is now accessible to nationaland international researchers. This report describes major features of the HR Bio-TEM and the facility, along with overallworkflow of cryo-EM image data acquisition.
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참고문헌 (25)
참고문헌 신청
Aebi, U / 1987 / A glow discharge unit to render electron microscope grids and other surfaces hydrophilic / J Electron Microsc Tech 7:29~33
Bartesaghi, A / 2013 / Prefusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy / Nat Struct Mol Biol 20:1352~1357
Campbell, M. G / 2015 / 2. 4 Angstrom resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy / eLife 4:e06380
Cheung, M / 2013 / A method to achieve homogeneous dispersion of large transmembrane complexes within the holes of carbon films for electron cryomicroscopy / J Struct Biol 182:51~56
Danev, R / 2017 / Expanding the boundaries of cryo-EM with phase plates / Curr Opin Struct Biol 46:87~94
Bartesaghi, A / 2013 / Prefusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy / Nat Struct Mol Biol 20:1352~1357
Campbell, M. G / 2015 / 2. 4 Angstrom resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy / eLife 4:e06380
Cheung, M / 2013 / A method to achieve homogeneous dispersion of large transmembrane complexes within the holes of carbon films for electron cryomicroscopy / J Struct Biol 182:51~56
Danev, R / 2017 / Expanding the boundaries of cryo-EM with phase plates / Curr Opin Struct Biol 46:87~94
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