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논문 기본 정보
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- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제8권 제2호
- 발행연도
- 2004.1
- 수록면
- 145 - 149 (5page)
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초록· 키워드
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The expression of a fusion protein comprised of the B.
thuringiensis crystal protein, Cry1Ac, and enhanced
green fluorescent protein (EGFP) in Escherichia coli
XL1-blue was examined. Three recombinant plasmids
were transformed into E. coli XL1-blue and named as
ProAc/Ec, MuEGFP/Ec and ProMu-EGFP/Ec, respectively.
All transformants were observed by light and
fluorescence microscopy at mid-log phase. The expression
in E. coli transformants, ProMu-EGFP/Ec and
MuEGFP/Ec, exhibited bright enough fluorescence to
be observed. Furthermore, ProMu-EGFP/Ec produced
fluorescent inclusions, which may have been
recombinant crystals between EGFP and Cry1Ac
while MuEGFP/Ec expressed soluble EGFP in cell. In
SDS-PAGE, ProAc/Ec had 130 kDa crystal protein
band and MuEGFP/Ec had thick 27 kDa EGFP band.
However, ProMu-EGFP/Ec had about 150 kDa fusion
protein band. Accordingly, these results indicated that
a fusion protein between the B. thuringiensis crystal
protein and a foreign protein under the lacZ promoter
was successfully expressed as granular structure in E.
coli. It is suggested that the E. coli expression system
by N-terminal fusion of B. thuringiensis crystal protein
may be useful as excellent means for fusion expression
and characterization of B. thuringiensis fusion crystal
protein.
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참고문헌 (27)
참고문헌 신청
/ 1970 / Cleavage of structural proteins duringthe assembly of the head of bacteriophage T4 : 680 ~ 227 685
/ 1972 / Nonchromo-somal antibiotic resistance in bacteria Genetic transforma-tion of Escherichia coli by R-factor DNA : 2110 ~ 69 2114
/ 1983 / Two types of entomocidaltoxins in the parasporal crystals of Bacillus thuringiensiskurstaki : 223 ~ 241
/ 1984 / A toxic fragment from the entomocidal crys-tal protein of Bacillus thuringiensis : 611 ~ 619
/ 1985 / Characterized full-lengthand truncated plasmid clones of the crystal protein of Bacil-lus thuringiensis subsp : 289 ~ 300
/ 1972 / Nonchromo-somal antibiotic resistance in bacteria Genetic transforma-tion of Escherichia coli by R-factor DNA : 2110 ~ 69 2114
/ 1983 / Two types of entomocidaltoxins in the parasporal crystals of Bacillus thuringiensiskurstaki : 223 ~ 241
/ 1984 / A toxic fragment from the entomocidal crys-tal protein of Bacillus thuringiensis : 611 ~ 619
/ 1985 / Characterized full-lengthand truncated plasmid clones of the crystal protein of Bacil-lus thuringiensis subsp : 289 ~ 300
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