지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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To identify low temperature-induced genes of wheat chromosome substitution line 5D, suppression subtractive hybridization (SSH) was performed with mRNAs from leaf samples that treated with low temperature (4℃). A cDNA library was constructed using mRNA isolated from wheat chromosome substitution line 5D leaves treated with low temperature (4℃). The nucleotide and deduced amino acid sequences of the putative gene products were compared. wfr-9 and wfr-32 showed identity over 90% related to vernalization gene. Other two genes, wfr-77 and wfr-83 which is related to freezing-resistant gene have also identity over 90%. This result suggest that those genes may be transcribed into antifreeze proteins which are accumulated within leaf apoplasts, when wheat chromosome substitution line 5D is acclimated during low temperature treatment.
목차
등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
Cattivelli L / 1990 / Molecular cloning and characterization of cold-regulated genes in barley / Plant Physiol 93 : 1504 ~ 1510
Chun JU / 1998 / Genetic studies of antifreeze proteins and their correlation with winter survival in wheat / Euphytica 102 : 219 ~ 226
Diatchenko L / 1996 / Suppression subtractive hybridization: A method for generation differentially regulated or tissue-specific cDNA probes and libraries / Proc. Natl. Acad.
Fowler DB / 1996 / The regulatory role of vernalization in the expression of low-temperature induced genes in wheat and rye / Theor. Appl. Genet 93 : 554 ~ 559
Griffith M / 1992 / Antifreeze protein produced endogenously in winter rye leaves / Plant Physiol 100 : 593 ~ 595
Chun JU / 1998 / Genetic studies of antifreeze proteins and their correlation with winter survival in wheat / Euphytica 102 : 219 ~ 226
Diatchenko L / 1996 / Suppression subtractive hybridization: A method for generation differentially regulated or tissue-specific cDNA probes and libraries / Proc. Natl. Acad.
Fowler DB / 1996 / The regulatory role of vernalization in the expression of low-temperature induced genes in wheat and rye / Theor. Appl. Genet 93 : 554 ~ 559
Griffith M / 1992 / Antifreeze protein produced endogenously in winter rye leaves / Plant Physiol 100 : 593 ~ 595
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