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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 243 - 249 (7page)
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초록· 키워드
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Tissue engineered bone (TEB) can replace an autogenous bone graft requiring an secondary operation site as well as avoid complications like inflammation or infection from xenogenic or synthetic bone graft. Adult mesenchymal stem cells (MSC) for TEB are considered to have various ranges of differentiation capacity or multipotency by the donor site and age. This study examined the effect of age on proliferation capacity, differentiation capacity and bone morphogenetic protein-2 (BMP-2) responsiveness of human bone marrow stromal cells (hBMSC) according to the age. In addition,to evaluate the effect on enhancement for osteoblast differentiation, the hBMSC were treated with Trichostatin A (TSA) and 5-Azacitidine (5-AZC)which was HDAC inhibitors and methyltransferase inhibitors respectively affecting chromatin remodeling temporarily and reversibly. The young and old group of hBMSC obtained from the iliac crest from total 9 healthy patients, showed similar proliferation capacity. Cell surface markers such as CD34, CD45, CD90 and CD105 showed uniform expression regardless of age. However, the young group showed more prominent transdifferentiation capacity with adipogenic differentiation. The osteoblast differentiation capacity or BMP responsiveness was low and similar between young and old group. TSA and 5-AZC showed potential for enhancing the BMP effect on osteoblast differentiation by increasing the expression level of osteogenic master gene, such as DLX5, ALP. More study will be needed to determine the positive effect of the reversible function of HDAC inhibitors or methyltransferase inhibitors on enhancing the low osteoblast differentiation capacity of hBMSC.
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Atkinson SP / 2007 / Epigenetic control of cellular senescence in disease: opportunities for therapeutic intervention / Expert Rev Mol Med 9 : 1 ~ 26
Benetti R / 2007 / Telomere length regulates the epigenetic status of mammalian telomeres and subtelomeres / Nat Genet 39 : 243 ~ 250
Conget PA / 1999 / Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells / J Cell Physiol 181 : 67 ~ 73
Diefenderfer DL / 2003 / BMP responsiveness in human mesenchymal stem cells / onnect Tissue Res 44 (1) : 305 ~ 311
Fang H / 2007 / Effect of rhBMP-2 and osteogenic revulsants on proliferation and differentiation of bone marrow stromal cells in rats / J Huazhong Univ Sci Technolog Med Sci
Benetti R / 2007 / Telomere length regulates the epigenetic status of mammalian telomeres and subtelomeres / Nat Genet 39 : 243 ~ 250
Conget PA / 1999 / Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells / J Cell Physiol 181 : 67 ~ 73
Diefenderfer DL / 2003 / BMP responsiveness in human mesenchymal stem cells / onnect Tissue Res 44 (1) : 305 ~ 311
Fang H / 2007 / Effect of rhBMP-2 and osteogenic revulsants on proliferation and differentiation of bone marrow stromal cells in rats / J Huazhong Univ Sci Technolog Med Sci
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