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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Objectives: The purpose of this study was to determine the effect of barium on gene expression in differentiation of human mesenchymal stem cells into osteoblasts in vitro.
Methods: Human bone marrow stem cells were cultured for 0∼14 days in osteogenic differentiation medium with strontium chloride (SrCl₂) and barium chloride (BaCl₂). Alkaline phosphatase (ALP) activity staining was the method selected for measuring osteoblast differentiation. Total ribonucleic acid (RNA) was extracted after 1, 3, 7, and 14 days, and analysis of runt-related transcription factor 2/core-binding factor alpha 1 (Runx2/Cbfa1), bone morphogenetic protein-2 (BMP-2), and bone sialoprotein (BSP) gene expression was performed by real-time reverse transcriptase (RT)-polymerase chain reaction (PCR).
Results: Barium and strontium had a superior enhancing effect on cell proliferation when compared to cells cultured in media without strontium or barium. BaCl2 produced a 2-fold increase in the expression of Runx2/Cbfa1 at 14 days. SrCl₂(0.1∼0.3 mM) produced a 2-fold increase in the expression of Runx2/Cbfa1 at 14 days. Barium produced a 1.5-fold increase in the expression of BMP-2 on days 1 or 3. Expression of BSP was increased 1.5∼1.7- and 2-fold on days 1 and 14 by barium and strontium, respectively.
Conclusion: Barium-like strontium is considered one of the important factors in inducing mesenchymal stem cells to differentiate into osteoblasts with further enhancement on bone formation.
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참고문헌 (17)
참고문헌 신청
Banerjee C / 2001 / Differential regulation of the two principal Runx2/ Cbfa1 n-terminal isoforms in response to bone morphogenetic protein-2 during development of the osteo
Burge RT / 2003 / Methodology for estimating current and future burden of osteoporosis in state populations: application to Florida in 2000 through 2025 / Value Health 6 : 57
Chen D / 2004 / Bone morphogenetic proteins / Growth Factors 22 : 233 ~ 241
Cranney A / 2002 / Meta-analyses of therapies for postmenopausal osteoporosis. IX: Summary of meta-analyses of therapies for postmenopausal osteoporosis / Endocr Rev 23 : 570
Francis RM / 2006 / Calcium, vitamin D and involutional osteoporosis / Curr Opin Clin Nutr Metab Care 9 : 13 ~ 17
Burge RT / 2003 / Methodology for estimating current and future burden of osteoporosis in state populations: application to Florida in 2000 through 2025 / Value Health 6 : 57
Chen D / 2004 / Bone morphogenetic proteins / Growth Factors 22 : 233 ~ 241
Cranney A / 2002 / Meta-analyses of therapies for postmenopausal osteoporosis. IX: Summary of meta-analyses of therapies for postmenopausal osteoporosis / Endocr Rev 23 : 570
Francis RM / 2006 / Calcium, vitamin D and involutional osteoporosis / Curr Opin Clin Nutr Metab Care 9 : 13 ~ 17
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