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논문 기본 정보
- 자료유형
- 학술저널
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제6호
- 발행연도
- 2016.1
- 수록면
- 1,067 - 1,076 (10page)
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The gene encoding lipase (Lip98) from Aeromicrobium sp. SCSIO 25071 was cloned and functionally expressed in Escherichia coli. Lip98 amino acid sequence shares the highest (49%) identity to Rhodococcus jostii RHA1 lipase and contains a novel motif (GHSEG), which is different from other clusters in the lipase superfamily. The recombinant lipase was purified to homogeneity with Ni-NTA affinity chromatography. Lip98 showed an apparent molecular mass of 30 kDa on SDS gel. The optimal temperature and pH value for enzymatic activity were recorded at 30°C and 7.5, respectively. Lip98 exhibited high activity at low temperatures with 35% maximum activity at 0°C and good stability at temperatures below 35°C. Its calculated activation energy was 4.12 kcal/mol at the low temperature range of 15-30°C. Its activity was slightly affected by some metal ions such as K+, Ca2+, and Na+. The activity of Lip98 was increased by various organic solvents such as DMSO, ethanol, acetone, and hexane with the concentration of 30% (v/v) and retained more than 30% residual activity in neat organic solvent. The unique characteristics of Lip98 imply that it is a promising candidate for industrial application as a nonaqueous biocatalyst and food additive.
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참고문헌 (9)
참고문헌 신청
Romero MD / 2007 / A kinetic study of isoamyl acetate synthesis by immobilized lipasecatalyzed acetylation in n-hexane / J. Biotechnol 127:269~277
Sharma S / 2014 / Organic solvent tolerant lipases and applications / Scientific World J 2014:625258
Street G / 1977 / Handbook of enzyme biotechnology / Biochem. Educ 5:64
Tran DT / 2013 / Kinetics of transesterification of olive oil with methanol catalyzed by immobilized lipase derived from an isolated Burkholderia sp. strain / Bioresour. Technol 145:193~203
Treichel H / 2009 / A review on microbial lipases production / Food Bioprocess Technol 3:182~196
Sharma S / 2014 / Organic solvent tolerant lipases and applications / Scientific World J 2014:625258
Street G / 1977 / Handbook of enzyme biotechnology / Biochem. Educ 5:64
Tran DT / 2013 / Kinetics of transesterification of olive oil with methanol catalyzed by immobilized lipase derived from an isolated Burkholderia sp. strain / Bioresour. Technol 145:193~203
Treichel H / 2009 / A review on microbial lipases production / Food Bioprocess Technol 3:182~196
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