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논문 기본 정보
- 자료유형
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제3호
- 발행연도
- 2017.1
- 수록면
- 514 - 523 (10page)
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초록· 키워드
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Carbon catabolite repression is a crucial regulation mechanism in microorganisms, but its characteristic in Rhizopus is still unclear. We extracted a carbon regulation gene, cre, that encoded a carbon catabolite repressor protein (CRE) from Rhizopus stolonifer TP-02, and studied the regulation of CRE by real-time qPCR. CRE responded to glucose in a certain range, where it could significantly regulate part of the cellulase genes (eg, bg, and cbh2) without cbh1. In the comparison of the response of cre and four cellulase genes to carboxymethylcellulose sodium and a simple carbon source (lactose), the effect of CRE was only related to the concentration of reducing sugars. By regulating the reducing sugars to range from 0.4% to 0.6%, a glucose-containing medium with lactose as the inducer could effectively induce cellulases without the repression of CRE. This regulation method could potentially reduce the cost of enzymes produced in industries and provide a possible solution to achieve the largescale synthesis of cellulases.
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참고문헌 (24)
참고문헌 신청
Bischof R / 2013 / Comparative analysis of the Trichoderma reesei transcriptome during growth on the cellulase inducing substrates wheat straw and lactose / Biotechnol. Biofu
Chen S / 1992 / Novel inducers derived from starch for cellulase production by Trichoderma reesei / Process Biochem 27 : 327 ~ 334
Cubero B / 1994 / Two different, adjacent and divergent zinc finger binding sites are necessary for CREAmediated carbon catabolite repression in the proline gene cluster of
Damsteegt EL / 2016 / Storage by lyophilization - resulting RNA quality is tissue dependent / Anal. Biochem 511 : 92 ~ 96
Drysdale MR / 1993 / The Aspergillus niger carbon catabolite repressor encoding gene, creA / Gene 130 : 241 ~ 245
Chen S / 1992 / Novel inducers derived from starch for cellulase production by Trichoderma reesei / Process Biochem 27 : 327 ~ 334
Cubero B / 1994 / Two different, adjacent and divergent zinc finger binding sites are necessary for CREAmediated carbon catabolite repression in the proline gene cluster of
Damsteegt EL / 2016 / Storage by lyophilization - resulting RNA quality is tissue dependent / Anal. Biochem 511 : 92 ~ 96
Drysdale MR / 1993 / The Aspergillus niger carbon catabolite repressor encoding gene, creA / Gene 130 : 241 ~ 245
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