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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제5호
- 발행연도
- 2018.1
- 수록면
- 826 - 830 (5page)
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초록· 키워드
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A Cre/loxP-δ-integration system was developed to allow sequential and simultaneous integration of a multiple gene expression cassette in Saccharomyces cerevisiae. To allow repeated integrations, the reusable Candida glabrata MARKER (CgMARKER) carrying loxP sequences was used, and the integrated CgMARKER was efficiently removed by inducing Cre recombinase. The XYLP and XYLB genes encoding endoxylanase and β-xylosidase, respectively, were used as model genes for xylan metabolism in this system, and the copy number of these genes was increased to 15.8 and 16.9 copies/cell, respectively, by repeated integration. This integration system is a promising approach for the easy construction of yeast strains with enhanced metabolic pathways through multicopy gene expression.
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등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Murray AW / 1983 / Pedigree analysis of plasmid segregation in yeast / Cell 34:961~970
Romanos MA / 1992 / Foreign gene expression in yeast: a review / Yeast 8:423~488
Kim MD / 2001 / Enhanced production of anticoagulant hirudin in recombinant Saccharomyces cerevisiae by chromosomal ${\delta}$ -integration / J. Biotechnol. 85:41~48
Sakai A / 1990 / Integration of heterologous genes into the chromosome of Saccharomyces cerevisiae using a ${\delta}$ -sequence of yeast retrotransposon Ty / Appl. Microbiol. Biotechnol. 33:302~306
Mochizuki D / 1994 / Overexpression and secretion of cellulolytic enzymes by ${\delta}$ -sequences mediated multicopy integration of heterologous DNA sequences into the chromosomes of Saccharomyces cerevisiae / J. Ferment. Bioeng. 77:468~473
Romanos MA / 1992 / Foreign gene expression in yeast: a review / Yeast 8:423~488
Kim MD / 2001 / Enhanced production of anticoagulant hirudin in recombinant Saccharomyces cerevisiae by chromosomal
Sakai A / 1990 / Integration of heterologous genes into the chromosome of Saccharomyces cerevisiae using a
Mochizuki D / 1994 / Overexpression and secretion of cellulolytic enzymes by
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