지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제3호
- 발행연도
- 2017.1
- 수록면
- 624 - 632 (9page)
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초록· 키워드
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Penicillium expansum causes blue mold rot, a prevalent postharvest disease of pome fruit, and is also the main producer of the patulin. However, knowledge on the molecular mechanisms involved in this pathogen-host interaction remains largely unknown. In this work, a twodimensional gel electrophoresis-based proteomic approach was applied to probe changes in P. expansum 3.3703 cultivated in apple juice medium, which was used to mimic the in planta condition. The results showed that the pH value and reducing sugar content in the apple juice medium decreased whereas the patulin content increased with the growing of P. expansum. A total of 28 protein spots that were up-regulated in P. expansum when grown in apple juice medium were identified. Functional categorization revealed that the identified proteins were mainly related to carbohydrate metabolism, secondary metabolism, protein biosynthesis or degradation, and redox homeostasis. Remarkably, several induced proteins, including glucose dehydrogenase, galactose oxidase, and FAD-binding monooxygenase, which might be responsible for the observed medium acidification and patulin production, were also detected. Overall, the experimental results provide a comprehensive interpretation of the physiological and proteomic responses of P. expansum to the host plant environment, and future functional characterization of the identified proteins will deepen our understanding of fungi-host interactions.
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참고문헌 (22)
참고문헌 신청
Andersen B / 2004 / Penicillium expansum: consistent production of patulin, chaetoglobosins, and other secondary metabolites in culture and their natural occurrence in fruit
Ballester A / 2015 / Genome, transcriptome, and functional analyses of Penicillium expansum provide new insights into secondary metabolism and pathogenicity / Mol. Plant Micr
Barad S / 2014 / Accumulation of the mycotoxin patulin in the presence of gluconic acid contributes to pathogenicity of Penicillium expansum / Mol. Plant Microbe Interact 27
Cai H / 2014 / Biochemical and proteomic analysis of g
Crespo-Sempere A / 2011 / Proteome analysis of the fungus Aspergillus carbonarius under ochratoxin A producing conditions / Int. J. Food Microbiol 147 : 162 ~ 169
Ballester A / 2015 / Genome, transcriptome, and functional analyses of Penicillium expansum provide new insights into secondary metabolism and pathogenicity / Mol. Plant Micr
Barad S / 2014 / Accumulation of the mycotoxin patulin in the presence of gluconic acid contributes to pathogenicity of Penicillium expansum / Mol. Plant Microbe Interact 27
Cai H / 2014 / Biochemical and proteomic analysis of g
Crespo-Sempere A / 2011 / Proteome analysis of the fungus Aspergillus carbonarius under ochratoxin A producing conditions / Int. J. Food Microbiol 147 : 162 ~ 169
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