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논문 기본 정보

자료유형
학술저널
저자정보
Rocío Crystabel López-González (Universidad Autónoma de Querétaro) Yara Suhan Juárez-Campusano (Universidad Autónoma de Querétaro) José Luis Rodríguez-Chávez (Universidad Autónoma de Querétaro) Guillermo Delgado-Lamas (Universidad Nacional Autónoma de México) Sofía María Arvizu Medrano (Universidad Autónoma de Querétaro) Ramón Álvar Martínez-Peniche (Universidad Autónoma de Querétaro) Juan Ramiro Pacheco-Aguilar (Universidad Autónoma de Querétaro)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal 제37권 제1호
발행연도
2021.2
수록면
24 - 35 (12page)

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Blue mold caused by Penicillium expansum is one of the most significant postharvest diseases of apples. Some microorganisms associated with the surface of ripening apples possess the ability to inhibit the growth of P. expansum. However, the existing literature about their colonization in the stages before ripening is not explored in depth. This study aims to characterize the antagonistic capacity of bacterial populations from five fruit development stages of ‘Royal Gala’ apples. The results have shown that the density of the bacterial populations decreases throughout the ripening stages of fruit (from 1.0 × 10<SUP>5</SUP> to 1.1 × 10¹ cfu/cm²). A total of 25 bacterial morphotypes (corresponding to five genera identified by 16S RNA) were differentiated in which Bacillus stood out as a predominant genus. In the in vitro antagonism tests, 10 Bacillus strains (40%) inhibited the mycelial growth of P. expansum from 30.1% to 60.1%, while in fruit bioassays, the same strains reduced the fruit rot ranging from 12% to 66%. Moreover, the bacterial strains with antagonistic activity increased in the ripening fruit stage. B. subtilis subsp. spiziennii M24 obtained the highest antagonistic activity (66.9% of rot reduction). The matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis revealed that bacteria with antagonistic activity produce anti-fungal lipopeptides from iturin and fengycin families.

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Materials and Methods
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