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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제12호
- 발행연도
- 2017.1
- 수록면
- 2,104 - 2,111 (8page)
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초록· 키워드
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A new series comprising phenylacetyl-homoserine lactones (HSLs), caffeoyl-HSL and feruloyl-HSL, was biologically synthesized using an artificial de novo biosynthetic pathway.
We developed an Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-HSLs from simple carbon sources. These artificial biosynthetic pathways contained the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. Finally, the yields for caffeoyl-HSL and feruloyl-HSL were 97.1 ± 10.3 and 65.2 ± 5.7 mg/l, respectively, by tyrosine-overproducing E. coli with a L-methionine feeding strategy. In a quorum sensing (QS) competition assay, feruloyl-HSL and p-coumaroyl-HSL antagonized the QS receptor TraR in Agrobacterium tumefaciens NT1, whereas caffeoyl-HSL did not.
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참고문헌 (23)
참고문헌 신청
[학술지(정기간
Ahlgren NA / 2011 / Aryl-homoserine lactone quorum sensing in stem-nodulating photosynthetic bradyrhizobia / Proc. Natl. Acad. Sci. USA 108 : 7183 ~ 7188
Choi O / 2011 / Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in Escherichia coli / J. Ind. Microbiol. Biotechnol 38
Eibergen NR / 2015 / Potent and selective modulation of the RhlR quorum sensing receptor by using non-native ligands: an emerging target for virulence control in Pseudomonas
Galloway WR / 2011 / Quorum sensing in gram-negative bacteria: small-molecule modulation of AHL and AI-2quorum sensing pathways / Chem. Rev 111 : 28 ~ 67
Ahlgren NA / 2011 / Aryl-homoserine lactone quorum sensing in stem-nodulating photosynthetic bradyrhizobia / Proc. Natl. Acad. Sci. USA 108 : 7183 ~ 7188
Choi O / 2011 / Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in Escherichia coli / J. Ind. Microbiol. Biotechnol 38
Eibergen NR / 2015 / Potent and selective modulation of the RhlR quorum sensing receptor by using non-native ligands: an emerging target for virulence control in Pseudomonas
Galloway WR / 2011 / Quorum sensing in gram-negative bacteria: small-molecule modulation of AHL and AI-2quorum sensing pathways / Chem. Rev 111 : 28 ~ 67
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