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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제12호
- 발행연도
- 2017.1
- 수록면
- 2,165 - 2,172 (8page)
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초록· 키워드
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Lignocellulose is now a promising raw material for biofuel production. However, the lignin complex and crystalline cellulose require pretreatment steps for breakdown of the crystalline structure of cellulose for the generation of fermentable sugars. Moreover, several fermentation inhibitors are generated with sugar compounds, majorly furfural. The mitigation of these inhibitors is required for the further fermentation steps to proceed. Amino acids were investigated on furfural-induced growth inhibition in E. coli producing isobutanol. Glycine and serine were the most effective compounds against furfural. In minimal media, glycine conferred tolerance against furfural. From the IC50 value for inhibitors in the production media, only glycine could alleviate growth arrest for furfural, where 6 mM glycine addition led to a slight increase in growth rate and isobutanol production from 2.6 to 2.8 g/l under furfural stress. Overexpression of glycine pathway genes did not lead to alleviation. However, addition of glycine to engineered strains blocked the growth arrest and increased the isobutanol production about 2.3-fold.
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참고문헌 (22)
참고문헌 신청
Cantarella M / 2004 / Effect of inhibitors released during steam-explosion treatment of poplar wood on subsequent enzymatic hydrolysis and SSF / Biotechnol. Progress 20 : 200
Geddes RD / 2014 / Polyamine transporters and polyamines increase furfural tolerance during xylose fermentation with ethanologenic Escherichia coli strain LY180 / Appl. Envi
Glebes TY / 2014 / Genome-wide mapping of furfural tolerance genes in Escherichia coli / PLoS One 9 : e87540 ~
Heer D / 2008 / Identification of furfural as a key toxin in lignocellulosic hydrolysates and evolution of a tolerant yeast strain / Microb. Biotechnol 1 : 497 ~ 506
Hendriks ATWM / 2009 / Pretreatments to enhance the digestibility of lignocellulosic biomass / Bioresour. Technol 100 : 10 ~ 18
Geddes RD / 2014 / Polyamine transporters and polyamines increase furfural tolerance during xylose fermentation with ethanologenic Escherichia coli strain LY180 / Appl. Envi
Glebes TY / 2014 / Genome-wide mapping of furfural tolerance genes in Escherichia coli / PLoS One 9 : e87540 ~
Heer D / 2008 / Identification of furfural as a key toxin in lignocellulosic hydrolysates and evolution of a tolerant yeast strain / Microb. Biotechnol 1 : 497 ~ 506
Hendriks ATWM / 2009 / Pretreatments to enhance the digestibility of lignocellulosic biomass / Bioresour. Technol 100 : 10 ~ 18
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