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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제11호
- 발행연도
- 2017.1
- 수록면
- 2,044 - 2,051 (8page)
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초록· 키워드
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The main pathological hallmark of Alzheimer’s disease is the deposition of amyloid-beta (Aβ) peptides in the brain. Aβ has been widely used to mimic several aspects of Alzheimer’s disease. However, several characteristics of amyloid-induced Alzheimer’s disease pathology are not well established, especially in mice. The present study aimed to develop a new Alzheimer’s disease model by investigating how Aβ can be effectively aggregated using prokaryotes and eukaryotes. To express the Aβ42 complex in HEK293 cells, we cloned the Aβ42 region in a tandem repeat and incorporated the resulting construct into a eukaryotic expression vector. Following transfection into HEK293 cells via lipofection, cell viability assay and western blotting analysis revealed that exogenous Aβ42 can induce cell death and apoptosis. In addition, recombinant His-tagged Aβ42 was successfully expressed in Escherichia coli BL21 (DE3) and not only readily formed Aβ complexes, but also inhibited the proliferation of SH-SY5Y cells and E. coli. For in vivo testing, recombinant His-tagged Aβ42 solution (3 μg/ μl in 1× PBS containing 1 mM Ni2+) was injected stereotaxically into the left and right lateral ventricles of the brains of C57BL/6J mice (n = 8). Control mice were injected with 1× PBS containing 1 mM Ni2+ following the same procedure. Ten days after the sample injection, the Morris water maze test confirmed that exogenous Aβ caused an increase in memory loss.
These findings demonstrated that Ni2+ is capable of complexing the 50-kDa amyloid and that intracerebroventricular injection of Aβ42 can lead to cognitive impairment, thereby providing improved Alzheimer’s disease models.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Bose A / 2016 / Mitochondrial dysfunction in Parkinson’s disease / J. Neurochem 139 (Suppl 1) : 216 ~ 231
Bradford MM. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Cao LH / 2016 / Morphine, a potential antagonist of cisplatin cytotoxicity, inhibits cisplatin-induced apoptosis and suppression of tumor growth in nasopharyngeal carcinoma
Cui L / 2013 / Ferulic acid inhibits the transition of amyloid-beta42 monomers to oligomers but accelerates the transition from oligomers to fibrils / J. Alzheimers Dis 37 :
Du X / 2015 / Enterovirus 71 induces apoptosis of SHSY5Y human neuroblastoma cells through stimulation of endogenous microRNA let-7b expression / Mol. Med. Rep 12 : 953 ~ 95
Bradford MM. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Cao LH / 2016 / Morphine, a potential antagonist of cisplatin cytotoxicity, inhibits cisplatin-induced apoptosis and suppression of tumor growth in nasopharyngeal carcinoma
Cui L / 2013 / Ferulic acid inhibits the transition of amyloid-beta42 monomers to oligomers but accelerates the transition from oligomers to fibrils / J. Alzheimers Dis 37 :
Du X / 2015 / Enterovirus 71 induces apoptosis of SHSY5Y human neuroblastoma cells through stimulation of endogenous microRNA let-7b expression / Mol. Med. Rep 12 : 953 ~ 95
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